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Team:Johns Hopkins/Notebook
From 2008.igem.org
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=== GROUP 2: MATa Specific-promoters === | === GROUP 2: MATa Specific-promoters === | ||
| + | |||
| + | Status report by Allison and Nate | ||
| + | Part no.: BBa_K110008 | ||
| + | Part Description: MFA1 (L+R) | ||
| + | Part Location: in a labeled box, second shelf from the top, -20 | ||
| + | degrees C refrigerator next to front door | ||
| + | Date: 7/10/08 | ||
| + | PCR successful? Yes | ||
| + | Cloning of PCR product successful: in progress | ||
| + | Sequencing of cloned PCR product successful: not done | ||
| + | Joining of validated part to adjacent part(s) status: not done | ||
| + | Problems to be solved: to be determined | ||
| + | Current status of this part: PCR was being troubleshooted, appeared to | ||
| + | have good results with regular PCR protocol (not touchdown) in which | ||
| + | there was a constant annealing temperature of 55 degrees C - see gel | ||
| + | |||
| + | Status report by Allison and Nate | ||
| + | Part no.: BBa_K110016 | ||
| + | Part Description: Ste2 (R+L) | ||
| + | Part Location: in a labeled box, second shelf from the top, -20 | ||
| + | degrees C refrigerator next to front door | ||
| + | Date: 7/10/08 | ||
| + | PCR successful? Yes | ||
| + | Cloning of PCR product successful: in progress | ||
| + | Sequencing of cloned PCR product successful: not done | ||
| + | Joining of validated part to adjacent part(s) status: not done | ||
| + | Problems to be solved: to be determined | ||
| + | Current status of this part: Both PCR protocols (touchdown and second | ||
| + | PCR with constant annealing temperature) produced product of the | ||
| + | correct size. BBa_K110016 was used as a control in the second PCR with | ||
| + | BBa_K110008. | ||
=== GROUP 3 === | === GROUP 3 === | ||
Revision as of 16:37, 12 July 2008
Notebook
Contents |
Files
iGEM Groups V1.0
iGEM Groups V2.01
Important reminders and notes
[Can make general comments here, so they don't get lost in peoples e-mail boxes]
July-11: Primers for group 1 were delivered yesterday
July 11: Lab meeting at 7:30PM in the lab to go over miniprep protocol
Tuesday July 15: Lab meeting at 6:30PM with Jessica. Have status reports ready.
Status Reports
GROUP 1: Fluorescent Proteins
status report by: Ingrid (work done by James) Part no.: BBa_K110017 Part Description: yESapphire RtL Part Location (in build a genome lab): In James and Jasper's PCR product Box, Stainless Steel 4 degree PCR successful?; Yes Cloning of PCR product successful: Y/N Sequencing of cloned PCR product successful: Not done Joining of validated part to adjacent part(s) status: Not done Problems to be solved: The PCR of this part yielded a very large product Current status of this part:
status report by: Ingrid (work done by James) Part no.: BBa_K110010 Part Description: yESapphire LtR Part Location (in build a genome lab): In James and Jasper's PCR product Box, Stainless Steel 4 degree PCR successful?; Yes Cloning of PCR product successful: Not done Sequencing of cloned PCR product successful: No Joining of validated part to adjacent part(s) status: Not done Problems to be solved: The PCR of this part yielded a very large product Current status of this part:
GROUP 2: MATa Specific-promoters
Status report by Allison and Nate Part no.: BBa_K110008 Part Description: MFA1 (L+R) Part Location: in a labeled box, second shelf from the top, -20 degrees C refrigerator next to front door Date: 7/10/08 PCR successful? Yes Cloning of PCR product successful: in progress Sequencing of cloned PCR product successful: not done Joining of validated part to adjacent part(s) status: not done Problems to be solved: to be determined Current status of this part: PCR was being troubleshooted, appeared to have good results with regular PCR protocol (not touchdown) in which there was a constant annealing temperature of 55 degrees C - see gel
Status report by Allison and Nate Part no.: BBa_K110016 Part Description: Ste2 (R+L) Part Location: in a labeled box, second shelf from the top, -20 degrees C refrigerator next to front door Date: 7/10/08 PCR successful? Yes Cloning of PCR product successful: in progress Sequencing of cloned PCR product successful: not done Joining of validated part to adjacent part(s) status: not done Problems to be solved: to be determined Current status of this part: Both PCR protocols (touchdown and second PCR with constant annealing temperature) produced product of the correct size. BBa_K110016 was used as a control in the second PCR with BBa_K110008.
GROUP 3
GROUP 4
GROUP 5
etc....
