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Team:Johns Hopkins/Notebook/GROUP 1: Fluorescent Proteins
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== GROUP 1: Fluorescent Proteins == | == GROUP 1: Fluorescent Proteins == | ||
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Date: July 17 2008 | Date: July 17 2008 | ||
Revision as of 14:19, 25 July 2008
GROUP 1: Fluorescent Proteins
Date: July 17 2008 status report by: Ingrid Part no.: BBa_K110018 Part Description: mCherry: Part Location (in build a genome lab): In silver fridge by door PCR successful?; Yes PCR Products of mCherry and vYFP Cloning of PCR product successful: Inconclusive Venus YFP and mCherry Miniprep check via digest Sequencing of cloned PCR product successful: Not done Joining of validated part to adjacent part(s) status: Not done Problems to be solved: Had some extra unknown products, with unknown bands in the gel Current status of this part: Done with touchdown PCR and gel. Next step is to clone product.
Date: July 17 2008 status report by: Ingrid Part no.: BBa_K110019 Part Description: mCherry: Part Location (in build a genome lab): In silver fridge by door PCR successful?; Yes PCR Products of mCherry and vYFP Cloning of PCR product successful: Inconclusive Venus YFP and mCherry Miniprep check via digest Sequencing of cloned PCR product successful: No Joining of validated part to adjacent part(s) status: Not done Problems to be solved: Need to figure out if gel after digest shows correct products
Date: July 17 2008 status report by: Ingrid Part no.: BBa_K110020 Part Description: Venus Enhanced YFP Part Location (in build a genome lab): In silver fridge by door PCR successful?; Yes PCR Products of mCherry and vYFP Cloning of PCR product successful: inconclusive Venus YFP and mCherry Miniprep check via digest Sequencing of cloned PCR product successful: No Joining of validated part to adjacent part(s) status: Not done Problems to be solved: Had some extra unknown products, with unknown bands in the gel Current status of this part: Done with touchdown PCR and gel. Next step is to clone product.
Date: July 17 2008 status report by: Ingrid Part no.: BBa_K110021 Part Description: Venus Enhanced YFP Part Location (in build a genome lab): In silver fridge by door PCR successful?; Yes PCR Products of mCherry and vYFP Cloning of PCR product successful: Inconclusive Venus YFP and mCherry Miniprep check via digest Sequencing of cloned PCR product successful: No Joining of validated part to adjacent part(s) status: Not done Problems to be solved: Had some extra unknown products, with unknown bands in the gel Current status of this part: Done with touchdown PCR and gel. Next step is to clone product.
