GROUP 2: MATa Specific-promoters
Status report by Allison and Nate
Part no.: BBa_K110008
Part Description: MFA1 (L+R)
Part Location: in a labeled box, second shelf from the top, -20
degrees C refrigerator next to front door
Date: 7/10/08
PCR successful? Yes
BioBricks 8 and 16
BioBricks 8 and 16 Using Constant Annealing
Short 2Way Stop, Alpha Promoters, & Sapphire FP
Cloning of PCR product successful: in progress
Sequencing of cloned PCR product successful: not done
Joining of validated part to adjacent part(s) status: not done
Problems to be solved: to be determined
Current status of this part: PCR was being troubleshooted, appeared to
have good results with regular PCR protocol (not touchdown) in which
there was a constant annealing temperature of 55 degrees C - see gel
Status report by Allison and Nate
Part no.: BBa_K110016
Part Description: Ste2 (R+L)
Part Location: in a labeled box, second shelf from the top, -20
degrees C refrigerator next to front door
Date: 7/10/08
PCR successful? Yes
BioBricks 8 and 16
BioBricks 8 and 16 Using Constant Annealing
Short 2Way Stop, Alpha Promoters, & Sapphire FP
Cloning of PCR product successful: in progress
Sequencing of cloned PCR product successful: not done
Joining of validated part to adjacent part(s) status: not done
Problems to be solved: to be determined
Current status of this part: Both PCR protocols (touchdown and second
PCR with constant annealing temperature) produced product of the
correct size. BBa_K110016 was used as a control in the second PCR with
BBa_K110008.
Status report by Allison and Nate
Part no.: BBa_K110008
Part Description: MFA1 (L+R)
Part Location: same as above, plates are at 4 degrees refrigerator near front door
Date: 7/14/08
PCR successful? Yes
Cloning of PCR product of successful? There were mainly light blue colonies
(only a couple white colonies)
Sequencing of cloned PCR product successful: not done
Joining of validated part to adjacent part(s) status: not done
Problems to be solved: Ligation
Current status of this part: plates are at 4 degrees; another ligation/transformation
will be completed soon
Status report by Allison and Nate
Part no.: BBa_K110016
Part Description: Ste2 (R+L)
Part Location: in a labeled box, second shelf from the top, -20 degrees C refrigerator next to
front door; plates at 4 degrees
Date: 7/14/08
PCR successful? Yes
Cloning of PCR product successful: There were many blue colonies (similar to the plate of BB_K110008)
Sequencing of cloned PCR product successful: not done
Joining of validated part to adjacent part(s) status: not done
Problems to be solved: Ligation
Current status of this part: plates are at 4 degrees; another ligation/transformation
will be completed soon
Status report by Allison and Nate
Part no.: BBa_K110008
Part Description: MFA1 (L+R)
Part Location: same as above, plates are at 4 degrees refrigerator near front door
Date: 7/22/08
PCR successful? Yes
(http://baderlab.bme.jhu.edu:8888/moodle18/mod/data/view.php?d=4&mode=single&page=2
Cloning of PCR product of successful? Yes (approx 60 white colonies between two plates)
Sequencing of cloned PCR product successful: not done
Joining of validated part to adjacent part(s) status: not done
Problems to be solved:
Current status of this part: 12 mini preps and the restriction digestion were completed;
preparing to send 3 samples to be sequenced
(http://baderlab.bme.jhu.edu:8888/moodle18/mod/data/view.php?d=4&rid=1478)</b>
Status report by Allison and Nate
Part no.: BBa_K110016
Part Description: Ste2 (R+L)
Part Location: in a labeled box, second shelf from the top, -20 degrees C refrigerator next to
front door; plates at 4 degrees
Date: 7/22/08
PCR successful? Yes
Cloning of PCR product successful: Yes (approx 20 white colonies on one plate)
Joining of validated part to adjacent part(s) status: not done
Problems to be solved:
Current status of this part: 12 mini preps and the restriction digestion were completed;
preparing to send 3 samples to be sequenced
(http://baderlab.bme.jhu.edu:8888/moodle18/mod/data/view.php?d=4&rid=1478)
"
