Team:Johns Hopkins/Notebook/GROUP 3: Short two way stops

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GROUP 3: Short two way stops

 Date: 7/21/08
 status report by: James
 Part no.: BBa_K110011
 Part Description: Between-bud 27-W FRS2-C LtR
 Part Location (in build a genome lab): In James and Jasper's PCR product Box,
  Stainless Steel 4 degree
 PCR successful?; Yes
 Cloning of PCR product successful: Yes 
 [http://www.jhu.edu/iGEM/Group3:ShortTwo-wayStops/2008-7-22.Short%202Way%20Stop,%20Alpha%20Promoters,%20&%20Sapphire%20FP.Allison%20Suarez%20and%20Nate%20Sotuyo%20.html Short 2Way Stop, Alpha Promoters, & Sapphire FP]
 Sequencing of cloned PCR product successful: No
 Joining of validated part to adjacent part(s) status: Not done
 Problems to be solved:
 Current status of this part: Miniprep digestion yielded no product, PCR; ligation; 
 transformation; and miniprep will be repeated by this Wednesday (07/23/08)
 status report by: James
 Part no.: BBa_K110012
 Part Description: Between STE2-W and BST1-C LtR
 Part Location (in build a genome lab): In James and Jasper's PCR product Box, 
  Stainless Steel 4 degree
 PCR successful?; Yes
 Cloning of PCR product successful: Yes 
 [http://www.jhu.edu/iGEM/Group3:ShortTwo-wayStops/2008-7-22.Short%202Way%20Stop,%20Alpha%20Promoters,%20&%20Sapphire%20FP.Allison%20Suarez%20and%20Nate%20Sotuyo%20.html Short 2Way Stop, Alpha Promoters, & Sapphire FP]
 Sequencing of cloned PCR product successful: No
 Joining of validated part to adjacent part(s) status: Not done
 Problems to be solved:
 Current status of this part: Miniprep digestion yielded no product, PCR; ligation; 
 transformation; and miniprep will be repeated by this Wednesday (07/23/08)
 status report by: James
 Part no.: BBa_K110013
 Part Description: Between-SWP82-W and EMP47-C LtR
 Part Location (in build a genome lab): In James and Jasper's PCR product Box,
  Stainless Steel 4 degree
 PCR successful?; No
 Cloning of PCR product successful: No 
 Sequencing of cloned PCR product successful: No
 Joining of validated part to adjacent part(s) status: Not done
 Problems to be solved: The PCR of this part yielded a very large product
 Current status of this part: