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Team:Johns Hopkins/Notebook/GROUP 4: Long Two-way Stops & Mat(alpha) specific promotors
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(Difference between revisions)
(New page: {{JHU}} == GROUP 4: Long Two-way Stops & Mat(alpha) specific promotors == Date: 7/10/08 Status report by: Jaime Liu Part no.: BBa_K110001 Part Description: BBa_K110001 - Betwee...) |
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Part Location (in build a genome lab): In 4C fridge #2 | Part Location (in build a genome lab): In 4C fridge #2 | ||
PCR successful?; Y/N (link such as this)- Yes | PCR successful?; Y/N (link such as this)- Yes | ||
| - | + | [http://www.jhu.edu/iGEM/Group4:LongTwo-wayStopsANDMATalphaSpecificPromoters/2008-7-22.iGEM%20PCR%20experimentation%20on%20BBa_K110001%20and%20BBa_K110003.Jaime.html iGEM PCR experimentation on BBa_K110001 and BBa_K110003] | |
Cloning of PCR product successful: Y/N Yes | Cloning of PCR product successful: Y/N Yes | ||
Sequencing of cloned PCR product successful: Y/N No | Sequencing of cloned PCR product successful: Y/N No | ||
Revision as of 02:06, 23 July 2008
GROUP 4: Long Two-way Stops & Mat(alpha) specific promotors
Date: 7/10/08 Status report by: Jaime Liu Part no.: BBa_K110001 Part Description: BBa_K110001 - Between-bud 27-W FRS2-C + 200bp into each gene LtR Part Location (in build a genome lab): In 4C fridge #2 PCR successful?; Y/N (link such as this)- Yes [http://www.jhu.edu/iGEM/Group4:LongTwo-wayStopsANDMATalphaSpecificPromoters/2008-7-22.iGEM%20PCR%20experimentation%20on%20BBa_K110001%20and%20BBa_K110003.Jaime.html iGEM PCR experimentation on BBa_K110001 and BBa_K110003] Cloning of PCR product successful: Y/N Yes Sequencing of cloned PCR product successful: Y/N No Joining of validated part to adjacent part(s) status: Not Done Problems to be solved: Not really a problem, but need do a mini-Prep and sequence Current status of this part: All cloned and inoculated into 100uL in 96 well plate for sequencing- put in incubator on 7/15
Date: 7/10/08 Status report by: Jaime Liu Part no.: BBa_K110003 Part Description: BBa_K110003 - Between-SWP82-W and EMP47-C +200 into each gene LtR Part Location (in build a genome lab): In 4C fridge #2 PCR successful?; Y/N (link such as this)- Yes BBa_K110003: http://baderlab.bme.jhu.edu:8888/moodle18/mod/data/view.php?d=4&rid=1463 Cloning of PCR product successful: Y/N Yes Sequencing of cloned PCR product successful: Y/N No Joining of validated part to adjacent part(s) status: Not Done Problems to be solved: Not really a problem, but need do a mini-Prep and sequence Current status of this part: All cloned and inoculated into 100uL in 96 well plate for sequencing- put in incubator on 7/15
Date: 7/10/08 Status report by: Jaime Liu Part no.: BBa_K110005 Part Description: BBa_K110005 - MFalpha2 LtR Part Location (in build a genome lab): In 4C fridge #2 PCR successful?; Y/N (link such as this)- Yes BBa_K110005: http://baderlab.bme.jhu.edu:8888/moodle18/mod/data/view.php?d=4&rid=1470 Cloning of PCR product successful: Y/N Yes Sequencing of cloned PCR product successful: Y/N No Joining of validated part to adjacent part(s) status: Not Done Problems to be solved: Not really a problem, but need do a mini-Prep and sequence Current status of this part: All cloned and inoculated into 100uL in 96 well plate for sequencing- put in incubator on 7/15
Date: 7/10/08 Status report by: Jaime Liu Part no.: BBa_K110006 Part Description: BBa_K110006 - MFalpha1 LtR Part Location (in build a genome lab): In 4C fridge #2 PCR successful?; Y/N (link such as this)- Yes BBa_K110006: http://baderlab.bme.jhu.edu:8888/moodle18/mod/data/view.php?d=4&rid=1470 Cloning of PCR product successful: Y/N Yes Sequencing of cloned PCR product successful: Y/N No Joining of validated part to adjacent part(s) status: Not Done Problems to be solved: Not really a problem, but need do a mini-Prep and sequence Current status of this part: All cloned and inoculated into 100uL in 96 well plate for sequencing- put in incubator on 7/15
