Team:KULeuven/30 July 2008

From 2008.igem.org

(Difference between revisions)
(Lab Work)
(Wet Lab)
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=== Wet Lab ===
=== Wet Lab ===
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*letting ''Xba''I doing its job during the night, gave a positive outcome. These parts (B0015, J23032) were then cut with ''Eco''RI, separated on gel and purified. The folowing ligations were made: C0040+B0015, J23109+J23032, I712074+J23032, E0022+B0015.
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*Letting ''Xba''I doing its job during the night, gave a positive outcome. These parts (B0015, J23032) were then cut with ''Eco''RI, separated on gel and purified. The folowing ligations were made: C0040+B0015, J23109+J23032, I712074+J23032, E0022+B0015.
*R0084 + B0032 were separated on a gel, but there was no fragment of 145bp was obeserved => so no ligation
*R0084 + B0032 were separated on a gel, but there was no fragment of 145bp was obeserved => so no ligation
*Fluid cultures were prepared with the following parts: C0062, R0062, P1010, R0011, C0060, I712022.
*Fluid cultures were prepared with the following parts: C0062, R0062, P1010, R0011, C0060, I712022.
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*New digests with ''Xba''I were made with: B0015, B0032, J23022, J23032
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*New digests with ''Xba''I were made with: B0015, B0032, J23022, J23032.
=== Dry Lab ===
=== Dry Lab ===

Revision as of 14:57, 30 July 2008

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Contents

Lab Work

Wet Lab

  • Letting XbaI doing its job during the night, gave a positive outcome. These parts (B0015, J23032) were then cut with EcoRI, separated on gel and purified. The folowing ligations were made: C0040+B0015, J23109+J23032, I712074+J23032, E0022+B0015.
  • R0084 + B0032 were separated on a gel, but there was no fragment of 145bp was obeserved => so no ligation
  • Fluid cultures were prepared with the following parts: C0062, R0062, P1010, R0011, C0060, I712022.
  • New digests with XbaI were made with: B0015, B0032, J23022, J23032.

Dry Lab

Modeling

Wiki

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Some bugs have been fixed in notebook.

Started making div stuff for homepage in case Imperial College's style is wanted.

Remarks