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Team:KULeuven/31 July 2008
From 2008.igem.org
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*A gel was run with the parts that were cut with ''Xba''I during the night. the result was: B0015 cut, J23022 not cut, B0032 cut, J23032 cut. The positive parts were cut with ''Eco''RI en those were again separated on gel, followed by purification. The following ligations were performed: J23100+B0032, R0084+B0032. | *A gel was run with the parts that were cut with ''Xba''I during the night. the result was: B0015 cut, J23022 not cut, B0032 cut, J23032 cut. The positive parts were cut with ''Eco''RI en those were again separated on gel, followed by purification. The following ligations were performed: J23100+B0032, R0084+B0032. | ||
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| + | *Miniprep of the following parts was performed: R0011, P1010, C0062, I712022, C0060, R0062. We also nanodropped them and digested them with ''Spe''I. The ''Spe''I of La Roche is empty, so we used the enzyme of NEB. Because ''EcoR''I doesn't cut very well in the buffer of ''Spe''I (buffer 2), we began to purifie it by ethanol-precipitation. We put it overnight in -20°C. | ||
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| + | *Competent TOP10 cells were transformed by heat shock with the ligation products that came out the overnight ligation. | ||
=== Dry Lab === | === Dry Lab === | ||
Revision as of 19:43, 31 July 2008
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Contents |
Lab Work
Wet Lab
- A gel was run with the parts that were cut with XbaI during the night. the result was: B0015 cut, J23022 not cut, B0032 cut, J23032 cut. The positive parts were cut with EcoRI en those were again separated on gel, followed by purification. The following ligations were performed: J23100+B0032, R0084+B0032.
- Miniprep of the following parts was performed: R0011, P1010, C0062, I712022, C0060, R0062. We also nanodropped them and digested them with SpeI. The SpeI of La Roche is empty, so we used the enzyme of NEB. Because EcoRI doesn't cut very well in the buffer of SpeI (buffer 2), we began to purifie it by ethanol-precipitation. We put it overnight in -20°C.
- Competent TOP10 cells were transformed by heat shock with the ligation products that came out the overnight ligation.
Dry Lab
Modeling
Wiki
Worked on timer, keeping track on the time distance till the jamboree. Timer will be done tomorrow, after that the homepage will be taken care of.
