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Team:KULeuven/31 July 2008
From 2008.igem.org
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=== Wet Lab === | === Wet Lab === | ||
| - | *A gel was run with the parts that were cut with ''Xba''I during the night. the result was: B0015 cut, J23022 not cut, B0032 cut, J23032 cut. The positive parts were cut with ''Eco''RI en those were again separated on gel. gave a positive result en were purified. | + | *A gel was run with the parts that were cut with ''Xba''I during the night. the result was: B0015 cut, J23022 not cut, B0032 cut, J23032 cut. The positive parts were cut with ''Eco''RI en those were again separated on gel. gave a positive result en were purified. The following ligations were performed: J23100+B0032, ROO84+B0032, C0060+B0015. |
=== Dry Lab === | === Dry Lab === | ||
Revision as of 13:18, 31 July 2008
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Contents |
Lab Work
Wet Lab
- A gel was run with the parts that were cut with XbaI during the night. the result was: B0015 cut, J23022 not cut, B0032 cut, J23032 cut. The positive parts were cut with EcoRI en those were again separated on gel. gave a positive result en were purified. The following ligations were performed: J23100+B0032, ROO84+B0032, C0060+B0015.
