Team:KULeuven/Freezer
From 2008.igem.org
(Difference between revisions)
(→Parts stored in the fridge in the lab (4°C)) |
(→Parts stored in the fridge in the lab (4°C)) |
||
Line 118: | Line 118: | ||
|[http://partsregistry.org/Part:BBa_R0084 R0084]||pSB1A2||OmpF promoter||+/- 40||32.0||14||July 23 | |[http://partsregistry.org/Part:BBa_R0084 R0084]||pSB1A2||OmpF promoter||+/- 40||32.0||14||July 23 | ||
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
- | |[http://partsregistry.org/Part:BBa_R1052 R1052]||pSB1K0||434cI regulated promoter||+/- 15||27.5||60|| | + | |[http://partsregistry.org/Part:BBa_R1052 R1052]||pSB1K0||434cI regulated promoter||+/- 15||27.5||60||Sept 05 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
|[http://partsregistry.org/Part:BBa_C0060 C0060]||pSB1A2||Lactonase||30||155.3||54||July 31 | |[http://partsregistry.org/Part:BBa_C0060 C0060]||pSB1A2||Lactonase||30||155.3||54||July 31 | ||
Line 146: | Line 146: | ||
|[http://partsregistry.org/Part:BBa_I714074 I714074]||pSB1A2||ribokey||+/- 48||469||68||August 30 | |[http://partsregistry.org/Part:BBa_I714074 I714074]||pSB1A2||ribokey||+/- 48||469||68||August 30 | ||
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
- | |[http://partsregistry.org/Part:BBa_R0040 R0040]||pSB1A2||tetR repressible promotor||+/- 27||74.6||45|| | + | |[http://partsregistry.org/Part:BBa_R0040 R0040]||pSB1A2||tetR repressible promotor||+/- 27||74.6||45||Sept 01 |
|} | |} | ||
Line 165: | Line 165: | ||
!width=90| '''Date''' | !width=90| '''Date''' | ||
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
- | |[http://partsregistry.org/Part:BBa_C0062 C0062]+[http://partsregistry.org/Part:BBa_B0015 B0015]||pSB1AK3||Ligation for Cell Death||+/- 46||124.8|| | + | |[http://partsregistry.org/Part:BBa_C0062 C0062]+[http://partsregistry.org/Part:BBa_B0015 B0015]||pSB1AK3||Ligation for Cell Death||+/- 46||124.8||A1||August 08 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
- | |[http://partsregistry.org/Part:BBa_C0056 C0056]+[http://partsregistry.org/Part:BBa_B0015 B0015]||pSB1A2||Ligation for Memory||+/- 46||276.7|| | + | |[http://partsregistry.org/Part:BBa_C0056 C0056]+[http://partsregistry.org/Part:BBa_B0015 B0015]||pSB1A2||Ligation for Memory||+/- 46||276.7||D5||Sept 05 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
- | |[http://partsregistry.org/Part:BBa_C0061 C0061]+[http://partsregistry.org/Part:BBa_B0015 B0015]||pSB1A2||Ligation for Inverter||+/- 46||159.1|| | + | |[http://partsregistry.org/Part:BBa_C0061 C0061]+[http://partsregistry.org/Part:BBa_B0015 B0015]||pSB1A2||Ligation for Inverter||+/- 46||159.1||D6||Sept 05 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
- | |[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_E0240 E0240]||pSB1A2||Ligation for Output||+/- 46||281.9|| | + | |[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_E0240 E0240]||pSB1A2||Ligation for Output||+/- 46||281.9||D7||Sept 05 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
- | |[http://partsregistry.org/Part:BBa_B0014 B0014]+[http://partsregistry.org/Part:BBa_B0033 B0033]||pSB1A2||Ligation for Cell Death||+/- 46||118.1|| | + | |[http://partsregistry.org/Part:BBa_B0014 B0014]+[http://partsregistry.org/Part:BBa_B0033 B0033]||pSB1A2||Ligation for Cell Death||+/- 46||118.1||D8||Sept 05 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
- | |[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_J23066 J23066]||J23006 (Ap)||Ligation for Filter (new)||+/- 46||421.9|| | + | |[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_J23066 J23066]||J23006 (Ap)||Ligation for Filter (new)||+/- 46||421.9||D9||Sept 05 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
- | |[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_B0033 B0033]||pSB1A2||Ligation for Memory||+/- 46||81.2|| | + | |[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_B0033 B0033]||pSB1A2||Ligation for Memory||+/- 46||81.2||E1||Sept 05 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
- | |[http://partsregistry.org/Part:BBa_R0053 R0053]+[http://partsregistry.org/Part:BBa_P0152 P0152]||pSB1A2||Ligation for Memory||+/- 46||109.5|| | + | |[http://partsregistry.org/Part:BBa_R0053 R0053]+[http://partsregistry.org/Part:BBa_P0152 P0152]||pSB1A2||Ligation for Memory||+/- 46||109.5||E2||Sept 05 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
- | |[http://partsregistry.org/Part:BBa_E0022 E0022]+[http://partsregistry.org/Part:BBa_B0015 B0015]||pSB1AK3||Ligation for Output||+/- 46||151.8|| | + | |[http://partsregistry.org/Part:BBa_E0022 E0022]+[http://partsregistry.org/Part:BBa_B0015 B0015]||pSB1AK3||Ligation for Output||+/- 46||151.8||A5||August 08 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
- | |[http://partsregistry.org/Part:BBa_I712074 I712074]+[http://partsregistry.org/Part:BBa_J23032 J23032]||BBa_J23006 (Ap)||Ligation for Filter||+/- 46||89.9|| | + | |[http://partsregistry.org/Part:BBa_I712074 I712074]+[http://partsregistry.org/Part:BBa_J23032 J23032]||BBa_J23006 (Ap)||Ligation for Filter||+/- 46||89.9||A3||August 08 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
- | |[http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032]||BBa_J23006 (Ap)||Ligation for Filter||+/- 46||94.4|| | + | |[http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032]||BBa_J23006 (Ap)||Ligation for Filter||+/- 46||94.4||A4||August 08 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
- | |[http://partsregistry.org/Part:BBa_J23116 J23116]+[http://partsregistry.org/Part:BBa_B0032 B0032]||pSB1A2||Ligation for Input||+/- 40||102.5|| | + | |[http://partsregistry.org/Part:BBa_J23116 J23116]+[http://partsregistry.org/Part:BBa_B0032 B0032]||pSB1A2||Ligation for Input||+/- 40||102.5||A8||August 25 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
- | |[http://partsregistry.org/Part:BBa_J23116 J23116]+[http://partsregistry.org/Part:BBa_B0032 B0032]||pSB1A2||Ligation for Input||+/- 35||96.2|| | + | |[http://partsregistry.org/Part:BBa_J23116 J23116]+[http://partsregistry.org/Part:BBa_B0032 B0032]||pSB1A2||Ligation for Input||+/- 35||96.2||B2||August 25 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
- | |[http://partsregistry.org/Part:BBa_K145015 K145015]+[http://partsregistry.org/Part:pSB1A2 pSB1A2]||pSB1A2||GFP with LVA tag||+/- 46||152.7|| | + | |[http://partsregistry.org/Part:BBa_K145015 K145015]+[http://partsregistry.org/Part:pSB1A2 pSB1A2]||pSB1A2||GFP with LVA tag||+/- 46||152.7||A2||August 08 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
- | |[http://partsregistry.org/Part:BBa_K145015 K145015]+[http://partsregistry.org/Part:pSB1A2 pSB1A2]||pSB1A2||GFP+LVA||+/- 48||257|| | + | |[http://partsregistry.org/Part:BBa_K145015 K145015]+[http://partsregistry.org/Part:pSB1A2 pSB1A2]||pSB1A2||GFP+LVA||+/- 48||257||E4||August 30 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
- | |[http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:pSB1A2 pSB1A2]||pSB1A2||ccdB||+/- 48||204.6|| | + | |[http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:pSB1A2 pSB1A2]||pSB1A2||ccdB||+/- 48||204.6||E3||August 30 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
- | |[http://partsregistry.org/Part:BBa_K145001 K145001]+[http://partsregistry.org/Part:pSB1A2 pSB1A2]||pSB1A2||T7 without UmuD||+/- 48||414.8|| | + | |[http://partsregistry.org/Part:BBa_K145001 K145001]+[http://partsregistry.org/Part:pSB1A2 pSB1A2]||pSB1A2||T7 without UmuD||+/- 48||414.8||E5||August 30 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
- | |[http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:pSB1A2 pSB1A2]||pSB1A2||ccdB||+/- 48||188.4|| | + | |[http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:pSB1A2 pSB1A2]||pSB1A2||ccdB||+/- 48||188.4||E6||August 30 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
- | |[http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:pSB1A2 pSB1A2]||pSB1A2||ccdB coding region||+/- 46||57.8|| | + | |[http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:pSB1A2 pSB1A2]||pSB1A2||ccdB coding region||+/- 46||57.8||B5||August 28 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
- | |[http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:pSB1A2 pSB1A2]||pSB1A2||ccdB coding region||+/- 46||72.1|| | + | |[http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:pSB1A2 pSB1A2]||pSB1A2||ccdB coding region||+/- 46||72.1||B8||August 28 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
- | |[http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:pSB1A2 pSB1A2]||pSB1A2||ccdB coding region||+/- 46||18.5|| | + | |[http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:pSB1A2 pSB1A2]||pSB1A2||ccdB coding region||+/- 46||18.5||B7||August 28 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
- | |[http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:pSB1A2 pSB1A2]||pSB1A2||ccdB coding region||+/- 46||23.3|| | + | |[http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:pSB1A2 pSB1A2]||pSB1A2||ccdB coding region||+/- 46||23.3||B6||August 28 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
- | |[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_B0032 B0032]||pSB1A2||Ligation for output||+/- 30||64.4|| | + | |[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_B0032 B0032]||pSB1A2||Ligation for output||+/- 30||64.4||A9||August 25 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
- | |[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_B0032 B0032]||pSB1A2||Ligation for Output||+/- 35||93.1|| | + | |[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_B0032 B0032]||pSB1A2||Ligation for Output||+/- 35||93.1||B1||August 25 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
- | |[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_J23022 J23022]||BBa_J23006 (Ap)||Ligation for Filter (old)||+/- 35||81.3|| | + | |[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_J23022 J23022]||BBa_J23006 (Ap)||Ligation for Filter (old)||+/- 35||81.3||B3||August 27 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
- | |[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_J23022 J23022]||BBa_J23006 (Ap)||Ligation for Filter (old)||+/- 35||86.3|| | + | |[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_J23022 J23022]||BBa_J23006 (Ap)||Ligation for Filter (old)||+/- 35||86.3||B4||August 27 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
- | |[http://partsregistry.org/Part:BBa_C0012 C0012]+[http://partsregistry.org/Part:BBa_B0015 B0015]||pSB1A2||Ligation for Inverter||+/- 40||226.9|| | + | |[http://partsregistry.org/Part:BBa_C0012 C0012]+[http://partsregistry.org/Part:BBa_B0015 B0015]||pSB1A2||Ligation for Inverter||+/- 40||226.9||C1||Sept 01 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
- | |[http://partsregistry.org/Part:BBa_C0012 C0012]+[http://partsregistry.org/Part:BBa_B0015 B0015]||pSB1A2||Ligation for Input||+/- 40||91.6|| | + | |[http://partsregistry.org/Part:BBa_C0012 C0012]+[http://partsregistry.org/Part:BBa_B0015 B0015]||pSB1A2||Ligation for Input||+/- 40||91.6||C2||Sept 01 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
- | |[http://partsregistry.org/Part:BBa_C0012 C0012]+[http://partsregistry.org/Part:BBa_B0015 B0015]||pSB1A2||Ligation for Input||+/- 40||143.8|| | + | |[http://partsregistry.org/Part:BBa_C0012 C0012]+[http://partsregistry.org/Part:BBa_B0015 B0015]||pSB1A2||Ligation for Input||+/- 40||143.8||C3||Sept 01 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
- | |[http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:BBa_B0015 B0015]||pSB1A2||Ligation for Cell Death||+/- 40||122.8|| | + | |[http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:BBa_B0015 B0015]||pSB1A2||Ligation for Cell Death||+/- 40||122.8||C4||Sept 01 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
- | |[http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:BBa_B0015 B0015]||pSB1A2||Ligation for Cell Death||+/- 40||119.8|| | + | |[http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:BBa_B0015 B0015]||pSB1A2||Ligation for Cell Death||+/- 40||119.8||C5||Sept 01 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
- | |[http://partsregistry.org/Part:BBa_K145015 K145015]+[http://partsregistry.org/Part:BBa_B0015 B0015]||pSB1A2||Ligation for Output||+/- 40|| | + | |[http://partsregistry.org/Part:BBa_K145015 K145015]+[http://partsregistry.org/Part:BBa_B0015 B0015]||pSB1A2||Ligation for Output||+/- 40||175.5||C7||Sept 01 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
- | |[http://partsregistry.org/Part: | + | |[http://partsregistry.org/Part:BBa_C0060 C0060]+[http://partsregistry.org/Part:BBa_B0015 B0015]||pSB1A2||Ligation for Reset||+/- 40||174.2||C8||Sept 01 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
- | |[http://partsregistry.org/Part:BBa_C0060 C0060]+[http://partsregistry.org/Part:BBa_B0015 B0015]||pSB1A2||Ligation for Reset||+/- 40|| | + | |[http://partsregistry.org/Part:BBa_C0060 C0060]+[http://partsregistry.org/Part:BBa_B0015 B0015]||pSB1A2||Ligation for Reset||+/- 40||188.5||C9||Sept 01 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
- | |[http://partsregistry.org/Part: | + | |[http://partsregistry.org/Part:BBa_K145001 K145001]+[http://partsregistry.org/Part:BBa_B0015 B0015]||pSB1A2||Ligation for Filter||+/- 40||242.9||D1||Sept 01 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
- | |[http://partsregistry.org/Part: | + | |[http://partsregistry.org/Part:BBa_K145201 K145201]||pSB1A2||Input (complete)||+/- 40||193.5||E7||Sept 06 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
- | |[http://partsregistry.org/Part: | + | |[http://partsregistry.org/Part:BBa_K145201 K145201]||pSB1A2||Input (complete)||+/- 40||200.7||E8||Sept 06 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
- | |[http://partsregistry.org/Part: | + | |[http://partsregistry.org/Part:BBa_K145001 K145001]+[http://partsregistry.org/Part:BBa_B0015 B0015]||pSB1A2||Ligation for Filter||+/- 40||183.1||D2||Sept 01 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
- | |[http://partsregistry.org/Part:BBa_R0011 R0011]+[http://partsregistry.org/Part:BBa_B0032 B0032]||pSB1A2||Ligation for Inverter||+/- 40|| | + | |[http://partsregistry.org/Part:BBa_R0011 R0011]+[http://partsregistry.org/Part:BBa_B0032 B0032]||pSB1A2||Ligation for Inverter||+/- 40||56.0||D3||Sept 01 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
- | |[http://partsregistry.org/Part: | + | |[http://partsregistry.org/Part:BBa_R0011 R0011]+[http://partsregistry.org/Part:BBa_B0032 B0032]||pSB1A2||Ligation for Inverter||+/- 40||106.3||D4||Sept 01 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
- | |[http://partsregistry.org/Part: | + | |[http://partsregistry.org/Part:BBa_C0040 C0040]+[http://partsregistry.org/Part:BBa_B0015 B0015]||pSB1A2||Ligation for Inverter||+/- 40||158.6||B9||Sept 01 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
- | |[http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_J23022 J23022]+[http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032]||BBa_J23006 (Ap)||Ligation for Filter (old)||+/- 20||58.3|| | + | |[http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_J23022 J23022]+[http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032]||BBa_J23006 (Ap)||Ligation for Filter (old)||+/- 20||150.2||A6||August 20 |
+ | |- style="background: #E0FFFF; text-align: center;" | ||
+ | |[http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_J23022 J23022]+[http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032]||BBa_J23006 (Ap)||Ligation for Filter (old)||+/- 20||58.3||A7||August 20 | ||
|} | |} | ||
Line 267: | Line 269: | ||
!width=90| '''Box''' | !width=90| '''Box''' | ||
!width=90| '''Date''' | !width=90| '''Date''' | ||
- | |||
- | |||
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
|[http://partsregistry.org/Part:BBa_B0015 B0015]|| ''Eco''R I||''Xba'' I||Vector||3303||11||11.7||1||July 31 | |[http://partsregistry.org/Part:BBa_B0015 B0015]|| ''Eco''R I||''Xba'' I||Vector||3303||11||11.7||1||July 31 | ||
+ | |- style="background: #E0FFFF; text-align: center;" | ||
+ | |[http://partsregistry.org/Part:BBa_B0015 B0015]|| ''Pst'' I||''Xba'' I||Insert||144||25||3.8||3||Sept 09 | ||
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
|[http://partsregistry.org/Part:BBa_B0015 B0015]|| ''Eco''R I||''Xba'' I||Vector||3303||10||13.6||2||August 26 | |[http://partsregistry.org/Part:BBa_B0015 B0015]|| ''Eco''R I||''Xba'' I||Vector||3303||10||13.6||2||August 26 | ||
Line 280: | Line 282: | ||
|[http://partsregistry.org/Part:BBa_B0033 B0033]|| ''Eco''R I||''Xba'' I||Vector||2092||20||10.9||31||August 27 | |[http://partsregistry.org/Part:BBa_B0033 B0033]|| ''Eco''R I||''Xba'' I||Vector||2092||20||10.9||31||August 27 | ||
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
- | |[http://partsregistry.org/Part:BBa_B0014 B0014]|| ''Eco''R I||''Spe'' I||Insert||110||20||1.3||50|| | + | |[http://partsregistry.org/Part:BBa_B0014 B0014]|| ''Eco''R I||''Spe'' I||Insert||110||20||1.3||50||Sept 02 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
|[http://partsregistry.org/Part:BBa_C0040 C0040]|| ''Eco''R I||''Spe'' I||Insert||677||18||7.1||6||July 24 | |[http://partsregistry.org/Part:BBa_C0040 C0040]|| ''Eco''R I||''Spe'' I||Insert||677||18||7.1||6||July 24 | ||
Line 289: | Line 291: | ||
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
|[http://partsregistry.org/Part:BBa_C0040 C0040]|| ''Spe'' I||''Pst'' I||Vector||2724||25||19.2||7||August 29 | |[http://partsregistry.org/Part:BBa_C0040 C0040]|| ''Spe'' I||''Pst'' I||Vector||2724||25||19.2||7||August 29 | ||
+ | |- style="background: #E0FFFF; text-align: center;" | ||
+ | |[http://partsregistry.org/Part:BBa_E0040 E0040]|| ''Spe'' I||''Pst'' I||Vector||2799||25||28.4||55||Sept 06 | ||
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
|[http://partsregistry.org/Part:BBa_K145015 K145015]|| ''Spe'' I||''Pst'' I||Vector||2823||25||18.8||33||August 29 | |[http://partsregistry.org/Part:BBa_K145015 K145015]|| ''Spe'' I||''Pst'' I||Vector||2823||25||18.8||33||August 29 | ||
Line 328: | Line 332: | ||
|[http://partsregistry.org/Part:BBa_K145001 K145001] (T7 poly)|| ''Eco''R I||''Spe'' I||Insert||2732||+/-8||14.1||41||August 12 | |[http://partsregistry.org/Part:BBa_K145001 K145001] (T7 poly)|| ''Eco''R I||''Spe'' I||Insert||2732||+/-8||14.1||41||August 12 | ||
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
- | |[http://partsregistry.org/Part:BBa_K145013 K145013] (aLuxI)|| ''Eco''R I||''Spe'' I||Insert||660||+/-25||20.8||53|| | + | |[http://partsregistry.org/Part:BBa_K145013 K145013] (aLuxI)|| ''Eco''R I||''Spe'' I||Insert||660||+/-25||20.8||53||Sept 05 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
- | |[http://partsregistry.org/Part:BBa_K145150 K145150] (promoter)|| ''Eco''R I||''Spe'' I||Insert||110||+/-25||9.2||54|| | + | |[http://partsregistry.org/Part:BBa_K145150 K145150] (promoter)|| ''Eco''R I||''Spe'' I||Insert||110||+/-25||9.2||54||Sept 05 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
|[http://partsregistry.org/Part:BBa_K145001 K145001] (T7 poly)|| ''Eco''R I||''Spe'' I||Insert||2732||+/-8||24.3||48||August 12 | |[http://partsregistry.org/Part:BBa_K145001 K145001] (T7 poly)|| ''Eco''R I||''Spe'' I||Insert||2732||+/-8||24.3||48||August 12 | ||
Line 337: | Line 341: | ||
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
|[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]|| ''Eco''R I||''Spe'' I||Plasmid||2056||24||27.7||47||August 25 | |[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]|| ''Eco''R I||''Spe'' I||Plasmid||2056||24||27.7||47||August 25 | ||
+ | |- style="background: #E0FFFF; text-align: center;" | ||
+ | |[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]|| ''Eco''R I||''Spe'' I||Plasmid||2056||24||24.3||52||Sept 05 | ||
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
|[http://partsregistry.org/Part:BBa_R0011 R0011]|| ''Eco''R I||''Spe'' I||Insert||72||20||8.5||13||July 30 | |[http://partsregistry.org/Part:BBa_R0011 R0011]|| ''Eco''R I||''Spe'' I||Insert||72||20||8.5||13||July 30 | ||
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
- | |[http://partsregistry.org/Part:BBa_E0240 E0240]|| ''Eco''R I||''Xba'' I||Vector||2955||28||25.2||42|| | + | |[http://partsregistry.org/Part:BBa_E0240 E0240]|| ''Eco''R I||''Xba'' I||Vector||2955||28||25.2||42||Sept 01 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
- | |[http://partsregistry.org/Part:BBa_E0040 E0040]|| ''Eco''R I||''Spe'' I||Insert||720||28||8.3||37|| | + | |[http://partsregistry.org/Part:BBa_E0040 E0040]|| ''Eco''R I||''Spe'' I||Insert||720||28||8.3||37||Sept 01 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
- | |[http://partsregistry.org/Part:BBa_C0056 C0056]|| ''Eco''R I||''Spe'' I||Insert||636||23||10.1||35|| | + | |[http://partsregistry.org/Part:BBa_C0056 C0056]|| ''Eco''R I||''Spe'' I||Insert||636||23||10.1||35||Sept 01 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
- | |[http://partsregistry.org/Part:BBa_C0061 C0061]|| ''Eco''R I||''Spe'' I||Insert||618||28||9.0||38|| | + | |[http://partsregistry.org/Part:BBa_C0061 C0061]|| ''Eco''R I||''Spe'' I||Insert||618||28||9.0||38||Sept 01 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
- | |[http://partsregistry.org/Part:BBa_P0152 P0152]|| ''Eco''R I||''Xba'' I||Vector||2930||28||23.2||43|| | + | |[http://partsregistry.org/Part:BBa_P0152 P0152]|| ''Eco''R I||''Xba'' I||Vector||2930||28||23.2||43||Sept 01 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
- | |[http://partsregistry.org/Part:BBa_P0353 P0353]|| ''Eco''R I||''Xba'' I||Vector||4000||28||15.3||45|| | + | |[http://partsregistry.org/Part:BBa_P0353 P0353]|| ''Eco''R I||''Xba'' I||Vector||4000||28||15.3||45||Sept 01 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
- | |[http://partsregistry.org/Part:BBa_J23066 J23066]|| ''Eco''R I||''Xba''I||Vector||2700||28||11.4||27|| | + | |[http://partsregistry.org/Part:BBa_J23066 J23066]|| ''Eco''R I||''Xba''I||Vector||2700||28||11.4||27||Sept 01 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
- | |[http://partsregistry.org/Part:BBa_B0015 B0015]|| ''Eco''R I||''Xba'' I||Vector||3303||20||12.6||4|| | + | |[http://partsregistry.org/Part:BBa_B0015 B0015]|| ''Eco''R I||''Xba'' I||Vector||3303||20||12.6||4||Sept 01 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
- | |[http://partsregistry.org/Part:BBa_B0034 B0034]|| ''Eco''R I||''Xba'' I||Vector||2091||28||15.6||44|| | + | |[http://partsregistry.org/Part:BBa_B0034 B0034]|| ''Eco''R I||''Xba'' I||Vector||2091||28||15.6||44||Sept1 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
|[http://partsregistry.org/Part:BBa_R0011 R0011]|| ''Eco''R I||''Spe'' I||Insert||72||20||7.5||14||July 30 | |[http://partsregistry.org/Part:BBa_R0011 R0011]|| ''Eco''R I||''Spe'' I||Insert||72||20||7.5||14||July 30 | ||
Line 368: | Line 374: | ||
|[http://partsregistry.org/Part:BBa_R0084 R0084]|| ''Eco''R I||''Spe'' I||Insert||125||25||6.1||9||July 29 | |[http://partsregistry.org/Part:BBa_R0084 R0084]|| ''Eco''R I||''Spe'' I||Insert||125||25||6.1||9||July 29 | ||
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
- | |[http://partsregistry.org/Part:BBa_R0053 R0053]|| ''Eco''R I||''Spe'' I||Insert||70||20||1.1||51|| | + | |[http://partsregistry.org/Part:BBa_R0053 R0053]|| ''Eco''R I||''Spe'' I||Insert||70||20||1.1||51||Sept 02 |
- | + | ||
- | + | ||
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
|[http://partsregistry.org/Part:BBa_I712074 I712074]|| ''Eco''R I||''Spe'' I||Insert||61||25||3.4||28||July 29 | |[http://partsregistry.org/Part:BBa_I712074 I712074]|| ''Eco''R I||''Spe'' I||Insert||61||25||3.4||28||July 29 | ||
Line 384: | Line 388: | ||
|[http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032]|| ''Eco''R I||''Xba'' I||Vector||3113||+/-20||8.3||78||August 14 | |[http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032]|| ''Eco''R I||''Xba'' I||Vector||3113||+/-20||8.3||78||August 14 | ||
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
- | |[http://partsregistry.org/Part:BBa_C0060 C0060]+[http://partsregistry.org/Part:BBa_B0015 B0015]|| ''Eco''R I||''Xba'' I||Vector||2986||+/-25||17.8||67|| | + | |[http://partsregistry.org/Part:BBa_C0060 C0060]+[http://partsregistry.org/Part:BBa_B0015 B0015]|| ''Eco''R I||''Xba'' I||Vector||2986||+/-25||17.8||67||Sept 03 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
- | |[http://partsregistry.org/Part:BBa_C0040 C0040]+[http://partsregistry.org/Part:BBa_B0015 B0015]|| ''Eco''R I||''Xba'' I||Vector||2857||+/-25||18.7||66|| | + | |[http://partsregistry.org/Part:BBa_C0040 C0040]+[http://partsregistry.org/Part:BBa_B0015 B0015]|| ''Eco''R I||''Xba'' I||Vector||2857||+/-25||18.7||66||Sept 03 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
- | |[http://partsregistry.org/Part:BBa_K145015 K145015]+[http://partsregistry.org/Part:BBa_B0015 B0015]|| ''Eco''R I||''Xba'' I||Vector||2956||+/-25||20.8||65|| | + | |[http://partsregistry.org/Part:BBa_K145015 K145015]+[http://partsregistry.org/Part:BBa_B0015 B0015]|| ''Eco''R I||''Xba'' I||Vector||2956||+/-25||20.8||65||Sept 03 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
- | |[http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:BBa_B0015 B0015]|| ''Eco''R I||''Xba'' I||Vector||2505||+/-25||18.1||64|| | + | |[http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:BBa_B0015 B0015]|| ''Eco''R I||''Xba'' I||Vector||2505||+/-25||18.1||64||Sept 03 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
- | |[http://partsregistry.org/Part:BBa_C0012 C0012]+[http://partsregistry.org/Part:BBa_B0015 B0015]|| ''Eco''R I||''Xba'' I||Vector||3327||+/-25||18.1||63|| | + | |[http://partsregistry.org/Part:BBa_C0012 C0012]+[http://partsregistry.org/Part:BBa_B0015 B0015]|| ''Eco''R I||''Xba'' I||Vector||3327||+/-25||18.1||63||Sept 03 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
|[http://partsregistry.org/Part:BBa_J23116 J23116]+[http://partsregistry.org/Part:BBa_B0032 B0032]|| ''Eco''R I||''Spe'' I||Insert||71||+/-28||2.5||76||August 25 | |[http://partsregistry.org/Part:BBa_J23116 J23116]+[http://partsregistry.org/Part:BBa_B0032 B0032]|| ''Eco''R I||''Spe'' I||Insert||71||+/-28||2.5||76||August 25 | ||
Line 402: | Line 406: | ||
|[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_B0032 B0032]|| ''Eco''R I||''Spe'' I||Insert||90||+/-28||2.6||75||August 25 | |[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_B0032 B0032]|| ''Eco''R I||''Spe'' I||Insert||90||+/-28||2.6||75||August 25 | ||
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
- | |[http://partsregistry.org/Part:BBa_R0011 R0011]+[http://partsregistry.org/Part:BBa_B0032 B0032]|| ''Eco''R I||''Spe'' I||Insert||90||+/-25||3.8||62|| | + | |[http://partsregistry.org/Part:BBa_R0011 R0011]+[http://partsregistry.org/Part:BBa_B0032 B0032]|| ''Eco''R I||''Spe'' I||Insert||90||+/-25||3.8||62||Sept 05 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
|[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_J23022 J23022]|| ''Eco''R I||''Spe'' I||Insert||311||+/-28||3.6||74||August 27 | |[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_J23022 J23022]|| ''Eco''R I||''Spe'' I||Insert||311||+/-28||3.6||74||August 27 |
Revision as of 10:50, 7 September 2008
dock/undock dropdown
Parts stored in the fridge in the lab (4°C)
MiniPrep Products: Parts |
---|
Part | Plasmid | Description | µl left | ng/µl | Box | Date |
---|---|---|---|---|---|---|
B0015 | pSB1AK3 | Terminator | +/- 10 | 112.3 | 7 | July 24 |
B0015 | pSB1AK3 | Terminator | +/- 20 | 130.3 | 8 | July 24 |
B0015 | pSB1AK3 | Terminator | +/- 40 | 303.1 | 9 | July 29 |
B0015 | pSB1AK3 | Terminator | +/- 20 | 213.6 | 10 | July 29 |
B0015 | pSB1AK3 | Terminator | +/- 48 | 311.0 | 11 | July 29 |
B0015 | pSB1AK3 | Terminator | +/- 30 | 214.0 | 12 | July 29 |
B0015 | pSB1AK3 | Terminator | +/- 40 | 209.5 | 13 | July 29 |
B0015 | pSB1AK3 | Terminator | +/- 40 | 63.0 | 6 | July 23 |
B0032 | pSB1A2 | RBS(medium) | +/- 5 | 51.0 | 57 | August 11 |
B0032 | pSB1A2 | RBS(medium) | +/- 20 | 47.2 | 55 | August 27 |
B0032 | pSB1A2 | RBS(medium) | +/- 40 | 27.0 | 56 | August 27 |
B0033 | pSB1AK3 | RBS | +/- 50 | 36.5 | 43 | August 27 |
B0033 | pSB1AK3 | RBS | +/- 23 | 38.4 | 44 | August 27 |
B0034 | pSB1A2 | RBS (efficiency 1) | +/- 20 | 73.8 | 47 | July 24 |
B0034 | pSB1A2 | RBS (efficiency 1) | +/- 30 | 95.1 | 48 | July 24 |
B0034 | pSB1A2 | RBS (efficiency 1) | +/- 40 | 104.0 | 49 | July 29 |
B0034 | pSB1AK3 | RBS | +/- 15 | 57 | 50 | August 27 |
B0034 | pSB1AK3 | RBS | +/- 50 | 29.6 | 51 | August 27 |
C0012 | pSB1A2 | LacI | +/- 30 | 133.1 | 20 | July 29 |
C0012 | pSB1A2 | LacI repressor | +/- 40 | 146.4 | 22 | July 29 |
C0012 | pSB1A2 | LacI repressor | +/- 40 | 203.1 | 21 | July 29 |
C0040 | pSB1A2 | TetR repressor | +/- 20 | 177.1 | 4 | July 24 |
C0040 | pSB1A2 | TetR repressor | +/- 30 | 161.7 | 5 | July 24 |
C0060 | pSB1A2 | lactonase production | +/- 48 | 84.2 | 53 | August 11 |
C0062 | pSB1A2 | LuxR repressor | +/- 48 | 76.2 | 38 | August 11 |
C0062 | pSB1AK3 | LuxR repressor | +/- 25 | 41.5 | 36 | August 27 |
C0062 | pSB1AK3 | LuxR repressor | +/- 50 | 31.7 | 37 | August 27 |
F1610 | pSB1AK3 | HSL Sender Device | +/- 50 | 57.6 | 16 | August 12 |
F1610 | pSB1AK3 | HSL Sender Device | +/- 40 | 53.1 | 17 | August 12 |
F1610 | pSB1AK3 | HSL Sender Device | +/- 50 | 41.4 | 18 | August 27 |
F1610 | pSB1AK3 | HSL Sender Device | +/- 20 | 31.5 | 19 | August 27 |
I712022 | BBa_J52017 (Km, Ap) | T7 polymerase | +/- 40 | 394.1 | 35 | July 31 |
I712074 | BBa_J52017 (Ap,Km) | T7 Promotor | +/- 42.5 | 208.2 | 32 | July 29 |
I712074 | BBa_J52017 (Ap,Km) | T7 Promotor | +/- 45 | 20.5 | 33 | August 27 |
I712074 | BBa_J52017 (Ap,Km) | T7 Promotor | +/- 45 | 63.9 | 34 | August 27 |
J23022 | BBa_J23006 (Ap) | 3d key+ terminator | +/- 40 | 160.7 | 26 | July 29 |
J23022 | BBa_J23006 (Ap) | 3d key+ terminator | +/- 46 | 308.3 | 27 | July 29 |
J23032 | BBa_J23006 (Ap) | 3d lock | +/- 35 | 117.4 | 23 | July 29 |
J23032 | BBa_J23006 (Ap) | 3d lock | +/- 39.5 | 144.8 | 24 | July 29 |
J23032 | BBa_J23006 (Ap) | 3d lock | +/- 48 | 129.1 | 25 | July 29 |
J23109 | BBa_J61002 (Ap) | Constitutive Promoter | +/- 46 | 375.1 | 28 | July 29 |
J23109 | BBa_J61002 (Ap) | Constitutive Promoter | +/- 48 | 99.2 | 30 | August 27 |
J23109 | BBa_J61002 (Ap) | Constitutive Promoter | +/- 48 | 69.2 | 31 | August 27 |
J23109 | BBa_J61002 (Ap) | Constitutive Promoter | +/- 48 | 404 | 29 | July 29 |
J23116 | BBa_J61002 (Ap) | Constitutive promoter | +/- 48 | 328.8 | 52 | August 08 |
R0011 | pSB1A2 | LacI regulated promoter | +/- 40 | 69.8 | 42 | July 29 |
R0011 | pSB1AK3 | LacI regulated promoter | +/- 50 | 30.8 | 40 | August 27 |
R0040 | pSB1A2 | tetR repressible promoter | +/- 48 | 37.9 | 46 | August 11 |
R0062 | pSB1A2 | LuxR regulated promoter | +/- 40 | 104.1 | 3 | July 31 |
R0084 | pSB1A2 | OmpF promoter | +/- 40 | 32.0 | 14 | July 23 |
R1052 | pSB1K0 | 434cI regulated promoter | +/- 15 | 27.5 | 60 | Sept 05 |
C0060 | pSB1A2 | Lactonase | 30 | 155.3 | 54 | July 31 |
C0062 | pSB1A2 | LuxR repressor/activator | ? | 159.4 | 35 | July 31 |
R0011 | pSB1A2 | lacI + pL promoter | ? | 68.0 | 39 | July 31 |
J23066 | J23006 | ribokey | +/- 45 | 541 | 58 | August 30 |
P0353 | pSB1AK3 | cII p22 van memory | +/- 45 | 406.9 | 1 | August 30 |
P0152 | pSB1A2 | cI 434 van memory | +/- 44 | 265.4 | 2 | August 30 |
E0240 | pSB1A2 | GFP generator | +/- 44 | 281 | 59 | August 30 |
C0056 | pSB1A2 | cI represor | +/- 45 | 419.8 | 64 | August 30 |
C0061 | pSB1A2 | autoinducer ASL synthetase, LuxI | +/- 45 | 338.6 | 65 | August 30 |
B0014 | pSB1AK3 | terminator | +/- 45 | 344.4 | 66 | August 30 |
E0040 | pSB1A2 | GFP | +/- 48 | 305.8 | 67 | August 30 |
R0053 | pSB1A2 | p22 cII regulated promotor | +/- 25 | 72.1 | 69 | August 30 |
I714074 | pSB1A2 | ribokey | +/- 48 | 469 | 68 | August 30 |
R0040 | pSB1A2 | tetR repressible promotor | +/- 27 | 74.6 | 45 | Sept 01 |
MiniPrep Products: Ligations |
---|
Parts | Plasmid | Description | µl left | ng/µl | Box | Date |
---|---|---|---|---|---|---|
C0062+B0015 | pSB1AK3 | Ligation for Cell Death | +/- 46 | 124.8 | A1 | August 08 |
C0056+B0015 | pSB1A2 | Ligation for Memory | +/- 46 | 276.7 | D5 | Sept 05 |
C0061+B0015 | pSB1A2 | Ligation for Inverter | +/- 46 | 159.1 | D6 | Sept 05 |
R0040+E0240 | pSB1A2 | Ligation for Output | +/- 46 | 281.9 | D7 | Sept 05 |
B0014+B0033 | pSB1A2 | Ligation for Cell Death | +/- 46 | 118.1 | D8 | Sept 05 |
R0040+J23066 | J23006 (Ap) | Ligation for Filter (new) | +/- 46 | 421.9 | D9 | Sept 05 |
R0040+B0033 | pSB1A2 | Ligation for Memory | +/- 46 | 81.2 | E1 | Sept 05 |
R0053+P0152 | pSB1A2 | Ligation for Memory | +/- 46 | 109.5 | E2 | Sept 05 |
E0022+B0015 | pSB1AK3 | Ligation for Output | +/- 46 | 151.8 | A5 | August 08 |
I712074+J23032 | BBa_J23006 (Ap) | Ligation for Filter | +/- 46 | 89.9 | A3 | August 08 |
J23109+J23032 | BBa_J23006 (Ap) | Ligation for Filter | +/- 46 | 94.4 | A4 | August 08 |
J23116+B0032 | pSB1A2 | Ligation for Input | +/- 40 | 102.5 | A8 | August 25 |
J23116+B0032 | pSB1A2 | Ligation for Input | +/- 35 | 96.2 | B2 | August 25 |
K145015+pSB1A2 | pSB1A2 | GFP with LVA tag | +/- 46 | 152.7 | A2 | August 08 |
K145015+pSB1A2 | pSB1A2 | GFP+LVA | +/- 48 | 257 | E4 | August 30 |
K145151+pSB1A2 | pSB1A2 | ccdB | +/- 48 | 204.6 | E3 | August 30 |
K145001+pSB1A2 | pSB1A2 | T7 without UmuD | +/- 48 | 414.8 | E5 | August 30 |
K145151+pSB1A2 | pSB1A2 | ccdB | +/- 48 | 188.4 | E6 | August 30 |
K145151+pSB1A2 | pSB1A2 | ccdB coding region | +/- 46 | 57.8 | B5 | August 28 |
K145151+pSB1A2 | pSB1A2 | ccdB coding region | +/- 46 | 72.1 | B8 | August 28 |
K145151+pSB1A2 | pSB1A2 | ccdB coding region | +/- 46 | 18.5 | B7 | August 28 |
K145151+pSB1A2 | pSB1A2 | ccdB coding region | +/- 46 | 23.3 | B6 | August 28 |
R0040+B0032 | pSB1A2 | Ligation for output | +/- 30 | 64.4 | A9 | August 25 |
R0040+B0032 | pSB1A2 | Ligation for Output | +/- 35 | 93.1 | B1 | August 25 |
R0040+J23022 | BBa_J23006 (Ap) | Ligation for Filter (old) | +/- 35 | 81.3 | B3 | August 27 |
R0040+J23022 | BBa_J23006 (Ap) | Ligation for Filter (old) | +/- 35 | 86.3 | B4 | August 27 |
C0012+B0015 | pSB1A2 | Ligation for Inverter | +/- 40 | 226.9 | C1 | Sept 01 |
C0012+B0015 | pSB1A2 | Ligation for Input | +/- 40 | 91.6 | C2 | Sept 01 |
C0012+B0015 | pSB1A2 | Ligation for Input | +/- 40 | 143.8 | C3 | Sept 01 |
K145151+B0015 | pSB1A2 | Ligation for Cell Death | +/- 40 | 122.8 | C4 | Sept 01 |
K145151+B0015 | pSB1A2 | Ligation for Cell Death | +/- 40 | 119.8 | C5 | Sept 01 |
K145015+B0015 | pSB1A2 | Ligation for Output | +/- 40 | 175.5 | C7 | Sept 01 |
C0060+B0015 | pSB1A2 | Ligation for Reset | +/- 40 | 174.2 | C8 | Sept 01 |
C0060+B0015 | pSB1A2 | Ligation for Reset | +/- 40 | 188.5 | C9 | Sept 01 |
K145001+B0015 | pSB1A2 | Ligation for Filter | +/- 40 | 242.9 | D1 | Sept 01 |
K145201 | pSB1A2 | Input (complete) | +/- 40 | 193.5 | E7 | Sept 06 |
K145201 | pSB1A2 | Input (complete) | +/- 40 | 200.7 | E8 | Sept 06 |
K145001+B0015 | pSB1A2 | Ligation for Filter | +/- 40 | 183.1 | D2 | Sept 01 |
R0011+B0032 | pSB1A2 | Ligation for Inverter | +/- 40 | 56.0 | D3 | Sept 01 |
R0011+B0032 | pSB1A2 | Ligation for Inverter | +/- 40 | 106.3 | D4 | Sept 01 |
C0040+B0015 | pSB1A2 | Ligation for Inverter | +/- 40 | 158.6 | B9 | Sept 01 |
R0084+J23022+J23109+J23032 | BBa_J23006 (Ap) | Ligation for Filter (old) | +/- 20 | 150.2 | A6 | August 20 |
R0084+J23022+J23109+J23032 | BBa_J23006 (Ap) | Ligation for Filter (old) | +/- 20 | 58.3 | A7 | August 20 |
Restriction Digests |
---|
Part | Enzyme 1 | Enzyme 2 | vector/insert | length (bp) | µl left | ng/µl | Box | Date |
---|---|---|---|---|---|---|---|---|
B0015 | EcoR I | Xba I | Vector | 3303 | 11 | 11.7 | 1 | July 31 |
B0015 | Pst I | Xba I | Insert | 144 | 25 | 3.8 | 3 | Sept 09 |
B0015 | EcoR I | Xba I | Vector | 3303 | 10 | 13.6 | 2 | August 26 |
B0032 | EcoR I | Xba I | Vector | 2092 | 5 | 17.6 | 25 | August 19 |
B0032 | EcoR I | Xba I | Vector | 2092 | 20 | 6.4 | 24 | August 29 |
B0033 | EcoR I | Xba I | Vector | 2092 | 20 | 10.9 | 31 | August 27 |
B0014 | EcoR I | Spe I | Insert | 110 | 20 | 1.3 | 50 | Sept 02 |
C0040 | EcoR I | Spe I | Insert | 677 | 18 | 7.1 | 6 | July 24 |
C0012 | Spe I | Pst I | Vector | 3192 | 25 | 16.4 | 32 | August 29 |
C0060 | Spe I | Pst I | Vector | 2853 | 25 | 16.3 | 18 | August 29 |
C0040 | Spe I | Pst I | Vector | 2724 | 25 | 19.2 | 7 | August 29 |
E0040 | Spe I | Pst I | Vector | 2799 | 25 | 28.4 | 55 | Sept 06 |
K145015 | Spe I | Pst I | Vector | 2823 | 25 | 18.8 | 33 | August 29 |
K145151 | Spe I | Pst I | Vector | 2370 | 25 | 14.5 | 34 | August 29 |
K145151 | Spe I | EcoR I | Vector | 2370 | 25 | 25.2 | 49 | August 29 |
K145001 | Spe I | Pst I | Vector | 4782 | 20 | 6.2 | 40 | August 29 |
C0061 | Spe I | Pst I | Vector | 2680 | 20 | 17.5 | 39 | August 31 |
C0056 | Spe I | Pst I | Vector | 2700 | 20 | 19.0 | 36 | August 31 |
C0060 | EcoR I | Spe I | Insert | 803 | 28 | 5.4 | 17 | August 12 |
C0060 | EcoR I | Spe I | Insert | 806 | 9 | 8.2 | 16 | August 01 |
C0062 | EcoR I | Spe I | Insert | 774 | 18 | 24.7 | 11 | August 01 |
C0062 | EcoR I | Spe I | Insert | 774 | 25 | 8.3 | 12 | August 27 |
C0062 | EcoR I | Spe I | Insert | 774 | 28 | 5.0 | 10 | August 12 |
F1610 | EcoR I | Xba I | Vector | 3956 | 26 | 4.5 | 30 | August 19 |
J23032 | EcoR I | Xba I | Vector | 3090 | 2 | 12.9 | 20 | July 30 |
J23032 | EcoR I | Xba I | Vector | 3090 | 45 | 1.3 | 21 | July 31 |
J23032 | EcoR I | Xba I | Vector | 3308 | 25 | 5.1 | 22 | August 07 |
J23100 | EcoR I | Spe I | Insert | 52 | 38.1 | 2.6 | 5 | July 24 |
J23109 | EcoR I | Spe I | Insert | 52 | 21 | 4.2 | 29 | July 29 |
J23116 | EcoR I | Spe I | Insert | 52 | +/-20 | 8.1 | 26 | August 12 |
K145001 (T7 poly) | EcoR I | Spe I | Insert | 2732 | +/-8 | 14.1 | 41 | August 12 |
K145013 (aLuxI) | EcoR I | Spe I | Insert | 660 | +/-25 | 20.8 | 53 | Sept 05 |
K145150 (promoter) | EcoR I | Spe I | Insert | 110 | +/-25 | 9.2 | 54 | Sept 05 |
K145001 (T7 poly) | EcoR I | Spe I | Insert | 2732 | +/-8 | 24.3 | 48 | August 12 |
pSB1A2 | EcoR I | Spe I | Plasmid | 2079 | 9 | 18.2 | 46 | August 01 |
pSB1A2 | EcoR I | Spe I | Plasmid | 2056 | 24 | 27.7 | 47 | August 25 |
pSB1A2 | EcoR I | Spe I | Plasmid | 2056 | 24 | 24.3 | 52 | Sept 05 |
R0011 | EcoR I | Spe I | Insert | 72 | 20 | 8.5 | 13 | July 30 |
E0240 | EcoR I | Xba I | Vector | 2955 | 28 | 25.2 | 42 | Sept 01 |
E0040 | EcoR I | Spe I | Insert | 720 | 28 | 8.3 | 37 | Sept 01 |
C0056 | EcoR I | Spe I | Insert | 636 | 23 | 10.1 | 35 | Sept 01 |
C0061 | EcoR I | Spe I | Insert | 618 | 28 | 9.0 | 38 | Sept 01 |
P0152 | EcoR I | Xba I | Vector | 2930 | 28 | 23.2 | 43 | Sept 01 |
P0353 | EcoR I | Xba I | Vector | 4000 | 28 | 15.3 | 45 | Sept 01 |
J23066 | EcoR I | XbaI | Vector | 2700 | 28 | 11.4 | 27 | Sept 01 |
B0015 | EcoR I | Xba I | Vector | 3303 | 20 | 12.6 | 4 | Sept 01 |
B0034 | EcoR I | Xba I | Vector | 2091 | 28 | 15.6 | 44 | Sept1 |
R0011 | EcoR I | Spe I | Insert | 72 | 20 | 7.5 | 14 | July 30 |
R0011 | EcoR I | Spe I | Insert | 72 | 25 | 2.1 | 15 | August 27 |
R0062 | EcoR I | Spe I | Insert | 55 | 27 | 7.8 | 23 | August 07 |
R0084 | EcoR I | Spe I | Insert | 125 | 33.4 | 3.8 | 8 | July 24 |
R0084 | EcoR I | Spe I | Insert | 125 | 25 | 6.1 | 9 | July 29 |
R0053 | EcoR I | Spe I | Insert | 70 | 20 | 1.1 | 51 | Sept 02 |
I712074 | EcoR I | Spe I | Insert | 61 | 25 | 3.4 | 28 | July 29 |
C0062+B0015 | EcoR I | Xba I | Vector | 4080 | +/-30 | 32.8 | 81 | August 14 |
E0022+B0015 | EcoR I | Xba I | Vector | 4086 | +/-30 | 14.8 | 80 | August 14 |
I712074+J23032 | EcoR I | Spe I | Insert | 112 | +/-30 | 3.9 | 79 | August 14 |
J23109+J23032 | EcoR I | Spe I | Insert | 101 | +/-30 | 4.0 | 77 | August 14 |
J23109+J23032 | EcoR I | Xba I | Vector | 3113 | +/-20 | 8.3 | 78 | August 14 |
C0060+B0015 | EcoR I | Xba I | Vector | 2986 | +/-25 | 17.8 | 67 | Sept 03 |
C0040+B0015 | EcoR I | Xba I | Vector | 2857 | +/-25 | 18.7 | 66 | Sept 03 |
K145015+B0015 | EcoR I | Xba I | Vector | 2956 | +/-25 | 20.8 | 65 | Sept 03 |
K145151+B0015 | EcoR I | Xba I | Vector | 2505 | +/-25 | 18.1 | 64 | Sept 03 |
C0012+B0015 | EcoR I | Xba I | Vector | 3327 | +/-25 | 18.1 | 63 | Sept 03 |
J23116+B0032 | EcoR I | Spe I | Insert | 71 | +/-28 | 2.5 | 76 | August 25 |
R0084+B0032 | EcoR I | Spe I | Insert | 144 | +/-20 | 0.9 | 72 | August 22 |
R0084+B0032 | EcoR I | Spe I | Insert | 144 | +/-30 | 2.9 | 71 | August 21 |
R0040+B0032 | EcoR I | Spe I | Insert | 90 | +/-28 | 2.6 | 75 | August 25 |
R0011+B0032 | EcoR I | Spe I | Insert | 90 | +/-25 | 3.8 | 62 | Sept 05 |
R0040+J23022 | EcoR I | Spe I | Insert | 311 | +/-28 | 3.6 | 74 | August 27 |
R0084+B0032 | EcoR I | Spe I | Insert | 144 | +/-28 | 2.4 | 73 | August 25 |
R0084+J23022 | EcoR I | Spe I | Insert | 365 | +/-20 | 6.9 | 70 | August 14 |
R0084+J23022+J23109+J23032 | EcoR I | Spe I | Insert | 432 | +/-30 | 4.6 | 68 | August 22 |
R0084+J23022+J23109+J23032 | EcoR I | Spe I | Insert | 432 | +/-30 | 3.7 | 69 | August 22 |
Things stored in freezer in lab (-20°C)
Miscellaneous |
---|
Description | Name | µl left | Concentration | Box | Date |
---|---|---|---|---|---|
Primer GFP-LVA | 1084 | 90 | 20 µM | 1 | August 01 |
Primer GFP-LVA | 1085 | 90 | 20 µM | 2 | August 01 |
dNTP mix | ATP, GTP, TTP, CTP | 70 | 10 mM | 3 | August 01 |
Primer GFP-LVA | 1084 | 500 | 100 µM | 4 | August 01 |
Primer GFP-LVA | 1085 | 500 | 100 µM | 5 | August 01 |
Primer Test | 1074 | 800 | 100 µM | 6 | August 07 |
Primer Test | 1075 | 780 | 100 µM | 7 | August 07 |
Primer T7-UmuD | 1076 | 560 | 100 µM | 8 | August 07 |
Primer T7-UmuD | 1077 | 477 | 100 µM | 9 | August 07 |
Primer T7-UmuD | 1078 | 467 | 100 µM | 10 | August 07 |
Primer T7-UmuD | 1079 | 45 | 100 µM | 11 | August 07 |
Primer Transduction | 1097 | 627 | 100 µM | 12 | August 07 |
Primer Transduction | 1098 | 710 | 100 µM | 13 | August 07 |
Primer Transduction | 1099 | 608 | 100 µM | 14 | August 07 |
Primer Anti-sense LuxI | 1100 | 522 | 100 µM | 15 | August 07 |
Primer Anti-sense LuxI | 1101 | 237 | 100 µM | 16 | August 07 |
Primer Test | 1074 | 100 | 20 µM | 17 | August 07 |
Primer Test | 1075 | 100 | 20 µM | 18 | August 07 |
Primer T7-UmuD | 1076 | 100 | 20 µM | 19 | August 07 |
Primer T7-UmuD | 1077 | 100 | 20 µM | 20 | August 07 |
Primer T7-UmuD | 1078 | 100 | 20 µM | 21 | August 07 |
Primer T7-UmuD | 1079 | 100 | 20 µM | 22 | August 07 |
Primer Transduction | 1097 | 100 | 20 µM | 23 | August 07 |
Primer Transduction | 1098 | 100 | 20 µM | 24 | August 07 |
Primer Transduction | 1099 | 100 | 20 µM | 25 | August 07 |
Primer Anti-sense LuxI | 1100 | 100 | 20 µM | 26 | August 07 |
Primer Anti-sense LuxI | 1101 | 100 | 20 µM | 27 | August 07 |
dNTP mix | ATP, GTP, TTP, CTP | 90 | 2 mM | 28 | August 19 |
Primer ccdB | 1127 | 100 | 100 µM | 29 | August 19 |
Primer ccdB | 1128 | 100 | 100 µM | 30 | August 19 |
Primer K145150 | 1129 | 100 | 100 µM | 31 | August 19 |
Primer K145150 | 1130 | 100 | 100 µM | 32 | August 19 |
Primer ccdB | 1127 | 90 | 20 µM | 33 | August 19 |
Primer ccdB | 1128 | 980 | 20 µM | 34 | August 19 |
Primer K145150 | 1129 | 990 | 1 µM | 35 | August 19 |
Primer K145150 | 1130 | 990 | 1 µM | 36 | August 19 |
dNTP mix | ATP, GTP, TTP, CTP | 990 | 2 µM | 37 | August 19 |