Team:KULeuven/Freezer

From 2008.igem.org

(Difference between revisions)
(Miniprep (stored in the fridge in the lab at 8°C))
(Miniprep (stored in the fridge in the lab at 8°C))
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|- style="background: #E0FFFF; text-align: center;"   
|- style="background: #E0FFFF; text-align: center;"   
|[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_J23066 J23066]+[http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032]||BBa_J23006 (Ap)||Ligation for Filter (new key)||+/- 45||394.3||G3||September 12
|[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_J23066 J23066]+[http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032]||BBa_J23006 (Ap)||Ligation for Filter (new key)||+/- 45||394.3||G3||September 12
 +
|- style="background: #E0FFFF; text-align: center;" 
 +
|[http://partsregistry.org/Part:BBa_K145013 K145013]+[http://partsregistry.org/Part:BBa_B0015 B0015]||pSB1A2 (Ap)||Ligation for Memory||+/- 50||141.3||H8||September 13
 +
|- style="background: #E0FFFF; text-align: center;" 
 +
|[http://partsregistry.org/Part:BBa_K145272 K145272||pSB1A2 (Ap)||Ligation for Filter test||+/- 50||145.9||H9||September 13
|}
|}

Revision as of 08:43, 13 September 2008

  dock/undock dropdown  


Miniprep (stored in the fridge in the lab at 8°C)

MiniPrep Products: Parts
Part Plasmid Description µl left ng/µl Box Date
B0015pSB1AK3Terminator+/- 10112.37July 24
B0015pSB1AK3Terminator+/- 20130.38July 24
B0015pSB1AK3Terminator+/- 40303.19July 29
B0015pSB1AK3Terminator+/- 20213.610July 29
B0015pSB1AK3Terminator+/- 48311.011July 29
B0015pSB1AK3Terminator+/- 30214.012July 29
B0015pSB1AK3Terminator+/- 40209.513July 29
B0015pSB1AK3Terminator+/- 4063.06July 23
B0032pSB1A2RBS(medium)+/- 551.057August 11
B0032pSB1A2RBS(medium)+/- 2047.255August 27
B0032pSB1A2RBS(medium)+/- 4027.056August 27
B0033pSB1AK3RBS+/- 5036.543August 27
B0033pSB1AK3RBS+/- 2338.444August 27
B0034pSB1A2RBS (efficiency 1)+/- 2073.847July 24
B0034pSB1A2RBS (efficiency 1)+/- 3095.148July 24
B0034pSB1A2RBS (efficiency 1)+/- 40104.049July 29
B0034pSB1AK3RBS+/- 155750August 27
B0034pSB1AK3RBS+/- 5029.651August 27
C0012pSB1A2LacI+/- 30133.120July 29
C0012pSB1A2LacI repressor+/- 40146.422July 29
C0012pSB1A2LacI repressor+/- 40203.121July 29
C0040pSB1A2TetR repressor+/- 20177.14July 24
C0040pSB1A2TetR repressor+/- 30161.75July 24
C0060pSB1A2lactonase production+/- 4884.253August 11
C0062pSB1A2LuxR repressor+/- 4876.238August 11
C0062pSB1AK3LuxR repressor+/- 2541.536August 27
F1610pSB1AK3HSL Sender Device+/- 5057.616August 12
F1610pSB1AK3HSL Sender Device+/- 4053.117August 12
F1610pSB1AK3HSL Sender Device+/- 5041.418August 27
F1610pSB1AK3HSL Sender Device+/- 2031.519August 27
I712022BBa_J52017 (Km, Ap)T7 polymerase+/- 40394.135July 31
I712074BBa_J52017 (Ap,Km)T7 Promotor+/- 42.5208.232July 29
I712074BBa_J52017 (Ap,Km)T7 Promotor+/- 4520.533August 27
I712074BBa_J52017 (Ap,Km)T7 Promotor+/- 4563.934August 27
J23022BBa_J23006 (Ap)3d key+ terminator+/- 40160.726July 29
J23022BBa_J23006 (Ap)3d key+ terminator+/- 46308.327July 29
J23032BBa_J23006 (Ap)3d lock+/- 35117.423July 29
J23032BBa_J23006 (Ap)3d lock+/- 39.5144.824July 29
J23032BBa_J23006 (Ap)3d lock+/- 48129.125July 29
J23109BBa_J61002 (Ap)Constitutive Promoter+/- 46375.128July 29
J23109BBa_J61002 (Ap)Constitutive Promoter+/- 4899.230August 27
J23109BBa_J61002 (Ap)Constitutive Promoter+/- 4869.231August 27
J23109BBa_J61002 (Ap)Constitutive Promoter+/- 4840429July 29
J23116BBa_J61002 (Ap)Constitutive promoter +/- 48328.852August 08
R0011pSB1A2LacI regulated promoter +/- 4069.842July 29
R0011pSB1AK3LacI regulated promoter+/- 5030.840August 27
R0040pSB1A2tetR repressible promoter+/- 4837.946August 11
R0062pSB1A2LuxR regulated promoter +/- 40104.13July 31
R0084pSB1A2OmpF promoter+/- 4032.014July 23
R1052pSB1K0434cI regulated promoter+/- 1527.560Sept 05
C0060pSB1A2Lactonase30155.354July 31
C0062pSB1A2LuxR repressor/activator?159.435July 31
R0011pSB1A2lacI + pL promoter?68.039July 31
J23066J23006ribokey+/- 4554158August 30
P0353pSB1AK3cII p22 van memory+/- 45406.91August 30
P0152pSB1A2cI 434 van memory+/- 44265.42August 30
E0240pSB1A2GFP generator+/- 4428159August 30
C0056pSB1A2cI represor+/- 45419.864August 30
C0061pSB1A2autoinducer ASL synthetase, LuxI+/- 45338.665August 30
B0014pSB1AK3terminator+/- 45344.466August 30
E0040pSB1A2GFP+/- 48305.867August 30
R0053pSB1A2p22 cII regulated promotor+/- 2572.169August 30
I714074pSB1A2ribokey+/- 4846968August 30
R0040pSB1A2tetR repressible promotor+/- 2774.645Sept 01


MiniPrep Products: Ligations
Parts Plasmid Description µl left ng/µl Box Date
C0062+B0015pSB1AK3Ligation for Cell Death+/- 46124.8A1August 08
C0056+B0015pSB1A2Ligation for Memory+/- 46276.7D5Sept 05
C0061+B0015pSB1A2Ligation for Inverter+/- 46159.1D6Sept 05
R0040+E0240pSB1A2Ligation for Output+/- 46281.9D7Sept 05
B0014+B0033pSB1A2Ligation for Cell Death+/- 46118.1D8Sept 05
R0040+J23066J23006 (Ap)Ligation for Filter (new)+/- 46421.9D9Sept 05
R0040+B0033pSB1A2Ligation for Memory+/- 4681.2E1Sept 05
R0053+P0152pSB1A2Ligation for Memory+/- 46109.5E2Sept 05
E0022+B0015pSB1AK3Ligation for Output+/- 46151.8A5August 08
I712074+J23032BBa_J23006 (Ap)Ligation for Filter+/- 4689.9A3August 08
J23109+J23032BBa_J23006 (Ap)Ligation for Filter+/- 4694.4A4August 08
J23116+B0032pSB1A2Ligation for Input+/- 40102.5A8August 25
J23116+B0032pSB1A2Ligation for Input+/- 3596.2B2August 25
K145015+pSB1A2pSB1A2GFP with LVA tag+/- 46152.7A2August 08
K145015+pSB1A2pSB1A2GFP+LVA+/- 48257E4August 30
K145151+pSB1A2pSB1A2ccdB+/- 48204.6E3August 30
K145001+pSB1A2pSB1A2T7 without UmuD+/- 48414.8E5August 30
K145151+pSB1A2pSB1A2ccdB+/- 48188.4E6August 30
K145151+pSB1A2pSB1A2ccdB coding region+/- 4657.8B5August 28
K145151+pSB1A2pSB1A2ccdB coding region+/- 4672.1B8August 28
K145151+pSB1A2pSB1A2ccdB coding region+/- 4618.5B7August 28
K145151+pSB1A2pSB1A2ccdB coding region+/- 4623.3B6August 28
K145015+pSB1A2pSB1A2GFP with LVA tag+/- 20104.5F6August 08
R0040+B0032pSB1A2Ligation for output+/- 3064.4A9August 25
R0040+B0032pSB1A2Ligation for Output+/- 3593.1B1August 25
R0040+J23022BBa_J23006 (Ap)Ligation for Filter (old)+/- 3581.3B3August 27
R0040+J23022BBa_J23006 (Ap)Ligation for Filter (old)+/- 3586.3B4August 27
C0012+B0015pSB1A2Ligation for Inverter+/- 40226.9C1Sept 01
C0012+B0015pSB1A2Ligation for Input+/- 4091.6C2Sept 01
C0012+B0015pSB1A2Ligation for Input+/- 40143.8C3Sept 01
K145151+B0015pSB1A2Ligation for Cell Death+/- 40122.8C4Sept 01
K145151+B0015pSB1A2Ligation for Cell Death+/- 40119.8C5Sept 01
K145015+B0015pSB1A2Ligation for Output+/- 40175.5C7Sept 01
C0060+B0015pSB1A2Ligation for Reset+/- 40174.2C8Sept 01
C0060+B0015pSB1A2Ligation for Reset+/- 40188.5C9Sept 01
K145001+B0015pSB1A2Ligation for Filter+/- 40242.9D1Sept 01
K145201pSB1A2Input: complete+/- 40193.5E7Sept 06
K145278pSB1A2part for filter+/- 42185.2G4Sept 12
K145201pSB1A2Input: complete+/- 40200.7E8Sept 06
K145253pSB1A2Inverter (old) - part 1+/- 40223.3E9Sept 06
K145253pSB1A2Inverter (old) - part 1+/- 40202.9F1Sept 06
K145251pSB1A2Reset (old): complete+/- 40168.6F2Sept 06
K145251pSB1A2Reset (old): complete+/- 40154.5F3Sept 06
K145205pSB1A2Output: complete+/- 40227.4F4Sept 06
K145205pSB1A2Output: complete+/- 40215.2F5Sept 06
K145254pSB1A2Ligation for Invertimer+/- 40137.6H3Sept 9
K145001+B0015pSB1A2Ligation for Filter+/- 40183.1D2Sept 01
R0011+B0032pSB1A2Ligation for Inverter+/- 4056.0D3Sept 01
R0011+B0032pSB1A2Ligation for Inverter+/- 40106.3D4Sept 01
C0040+B0015pSB1A2Ligation for Inverter+/- 40158.6B9Sept 01
C0040+B0015pSB1A2Ligation for Inverter+/- 40168.6G5Sept 9
K145150+BE0240pSB1A2Control for K145150 promotor+/- 40178.4G6Sept 9
K145150+pSB1A2pSB1A2Hybrid Promotor+/- 4097.6G7Sept 9
K145013+B0015pSB1A2Ligation for Inverter+/- 4089G8Sept 9
K145151+B0015pSB1A2Celldeath+/- 40122.8G9Sept 9
K145150+B0034pSB1A2Ligation for Celldeath+/- 3047.5H1Sept 9
R0040+P0353pSB1AK3Ligation for Memory+/- 10229.1H2Sept 9
R0084+J23022+J23109+J23032BBa_J23006 (Ap)Ligation for Filter (old)+/- 20150.2A6August 20
R0084+J23022+J23109+J23032BBa_J23006 (Ap)Ligation for Filter (old)+/- 2058.3A7August 20
R0040+J23066+J23109+J23032BBa_J23006 (Ap)Ligation for Filter (new key)+/- 45394.3G3September 12
K145013+B0015pSB1A2 (Ap)Ligation for Memory+/- 50141.3H8September 13
[http://partsregistry.org/Part:BBa_K145272 K145272pSB1A2 (Ap)Ligation for Filter test+/- 50145.9H9September 13

Digests (stored in the fridge in the lab at 8°C)

Restriction Digests: parts
Part Enzyme 1 Enzyme 2 vector/insert length (bp) µl left ng/µl Box Date
B0034 EcoR IXba IVector207525-58September 11
B0015 EcoR IXba IVector33031111.71July 31
B0015 Pst IXba IInsert144253.83Sept 09
B0015 EcoR IXba IVector33031013.62August 26
B0032 EcoR IXba IVector2092517.625August 19
B0032 EcoR IXba IVector2092206.424August 29
B0033 EcoR IXba IVector20922010.931August 27
B0014 EcoR ISpe IInsert110201.350Sept 02
C0040 EcoR ISpe IInsert677187.16July 24
C0012 Spe IPst IVector31922516.432August 29
C0060 Spe IPst IVector28532516.318August 29
C0040 Spe IPst IVector27242519.27August 29
E0040 Spe IPst IVector27992528.455Sept 06
K145015 Spe IPst IVector28232518.833August 29
K145151 Spe IPst IVector23702514.534August 29
K145151 Spe IEcoR IVector23702525.249August 29
K145001 Spe IPst IVector4782206.240August 29
C0061 Spe IPst IVector26802017.539August 31
C0056 Spe IPst IVector27002019.036August 31
C0060 EcoR ISpe IInsert803285.417August 12
C0060 EcoR ISpe IInsert80698.216August 01
C0062 EcoR ISpe IInsert7741824.711August 01
C0062 EcoR ISpe IInsert774258.312August 27
C0062 EcoR ISpe IInsert774285.010August 12
C0062 Pst IXba IInsert77140?56Sept 10
F1610 EcoR IXba IVector3956264.530August 19
J23032 EcoR IXba IVector3090212.920July 30
J23032 EcoR IXba IVector3090451.321July 31
J23032 EcoR IXba IVector3308255.122August 07
J23100EcoR ISpe IInsert5238.12.65July 24
J23109 EcoR ISpe IInsert52214.229July 29
J23116 EcoR ISpe IInsert52+/-208.126August 12
K145001 EcoR ISpe IInsert2732+/-814.141August 12
K145013 EcoR ISpe IInsert660+/-2520.853Sept 05
K145150 EcoR ISpe IInsert110+/-259.254Sept 05
K145151 Xba I/Vector240028?57August 12
K145001 EcoR ISpe IInsert2732+/-824.348August 12
pSB1A2 EcoR ISpe IPlasmid2079918.246August 01
pSB1A2 EcoR ISpe IPlasmid20562427.747August 25
pSB1A2 EcoR ISpe IPlasmid20562424.352Sept 05
R0011 EcoR ISpe IInsert72208.513July 30
E0240 EcoR IXba IVector29552825.242Sept 01
E0040 EcoR ISpe IInsert720288.337Sept 01
C0056 EcoR ISpe IInsert6362310.135Sept 01
C0061 EcoR ISpe IInsert618289.038Sept 01
P0152 EcoR IXba IVector29302823.243Sept 01
P0353 EcoR IXba IVector40002815.345Sept 01
J23066 EcoR IXbaIVector27002811.427Sept 01
B0015 EcoR IXba IVector33032012.64Sept 01
B0034 EcoR IXba IVector20912815.644Sept1
R0011 EcoR ISpe IInsert72207.514July 30
R0011 EcoR ISpe IInsert72252.115August 27
R0062 EcoR ISpe IInsert55277.823August 07
R0084 EcoR ISpe IInsert12533.43.88July 24
R0084 EcoR ISpe IInsert125256.19July 29
R0053 EcoR ISpe IInsert70201.151Sept 02
I712074 EcoR ISpe IInsert61253.428July 29


Restriction Digests: Ligations
Part Enzyme 1 Enzyme 2 vector/insert length (bp) µl left ng/µl Box Date
J23109+J23032 EcoR IXba IVector3350+/-30-F3September 11
C0062+B0015 EcoR IXba IVector4080+/-3032.8A1August 14
C0061+B0015 EcoR IXba IVector2800+/-3017.3A2Sept 06
R0040+J23066 EcoR ISpe IInsert411+/-306.6A3Sept 06
R0053+P0152 EcoR ISpe IInsert926+/-309.0A4Sept 06
B0014+B0033 EcoR ISpe IInsert132+/-306.1A5Sept 06
R0040+B0033 EcoR ISpe IInsert86+/-304.1A6Sept 06
C0056+B0015 EcoR IXba IVector3930+/-3012.8A7Sept 06
K145001+B0015 EcoR IXba IVector4915+/-3013.7A8Sept 06
K145150+B0034 SpeI Pst IVector2682+/-30?D5Sept 10
R0040+E0240 EcoR IXba IVector2981+/-3014.8A9Sept 06
E0022+B0015 EcoR IXba IVector4086+/-3014.8B1August 14
I712074+J23032 EcoR ISpe IInsert112+/-303.9B2August 14
J23109+J23032 EcoR ISpe IInsert101+/-304.0B3August 14
C0060+B0015 EcoR IXba IVector2986+/-2517.8B4Sept 03
C0040+B0015 EcoR IXba IVector2857+/-2518.7B5Sept 03
K145015+B0015 EcoR IXba IVector2956+/-2520.8B6Sept 03
K145151+B0015 EcoR IXba IVector2505+/-2518.1B7Sept 03
K145201 EcoR ISpe IInsert900+/-107.3D1Sept 07
K145205 EcoR IXba IVector3065+/-2026.9D2Sept 07
K145253 EcoR ISpe IInsert1410+/-2010.9D3Sept 07
K145251 EcoR Ixba IVector3133+/-2021.0D4Sept 07
K145258 EcoR ISpe IInsert852+/-30-F4Sept 11
K145254 EcoR IXba IVector2925+/-30?D7Sept 10
K145151+B0015 EcoR IXba IVector2505+/-30?D9Sept 10
K145150+E0240 EcoR IXba IVector3004+/-30?E1Sept 10
K145013+pSB1A2 Spe IPst IVector2682+/-30?E2Sept 10
C0012+B0015 EcoR IXba IVector3327+/-2518.1B8Sept 03
E0040+B0015 EcoR IXba IVector2919+/-30?D8Sept 10
J23116+B0032 EcoR ISpe IInsert71+/-282.5B9August 25
R0084+B0032 EcoR ISpe IInsert144+/-200.9C1August 22
R0084+B0032 EcoR ISpe IInsert144+/-302.9C2August 21
R0040+B0032 EcoR ISpe IInsert90+/-282.6C3August 25
R0040+P0353 EcoR IXba IVector4100+/-30?D6Sept 10
R0011+B0032 EcoR ISpe IInsert90+/-253.8C4Sept 05
R0040+J23022 EcoR ISpe IInsert311+/-283.6C5August 27
R0084+B0032 EcoR ISpe IInsert144+/-282.4C6August 25
R0084+J23022 EcoR ISpe IInsert365+/-206.9C7August 14
R0084+J23022+J23109+J23032 EcoR ISpe IInsert432+/-304.6C9August 21
R0084+J23022+J23109+J23032 EcoR ISpe IInsert432+/-303.7C8August 22

Things stored in freezer in lab (-20°C)

Miscellaneous
Description Name µl left Concentration Box Date
Primer GFP-LVA10849020 µM2August 01
Primer GFP-LVA10859020 µM4August 01
dNTP mixATP, GTP, TTP, CTP20010 mM81August 28
Primer GFP-LVA1084500100 µM1August 01
Primer GFP-LVA1085500100 µM3August 01
Primer Test1074800100 µM5August 07
Primer Test1075780100 µM7August 07
Primer T7-UmuD1076560100 µM9August 07
Primer T7-UmuD1077477100 µM11August 07
Primer T7-UmuD1078467100 µM13August 07
Primer T7-UmuD107945100 µM15August 07
Primer Transduction1097627100 µM17August 07
Primer Transduction1098710100 µM19August 07
Primer Transduction1099608100 µM21August 07
Primer Anti-sense LuxI1100522100 µM23August 07
Primer Anti-sense LuxI1101237100 µM25August 07
Primer Test107410020 µM6August 07
Primer Test107510020 µM8August 07
Primer T7-UmuD107610020 µM10August 07
Primer T7-UmuD107710020 µM12August 07
Primer T7-UmuD107810020 µM14August 07
Primer T7-UmuD107910020 µM16August 07
Primer Transduction109710020 µM18August 07
Primer Transduction109810020 µM20August 07
Primer Transduction109910020 µM22August 07
Primer Anti-sense LuxI110010020 µM24August 07
Primer Anti-sense LuxI110110020 µM26August 07
dNTP mixATP, GTP, TTP, CTP902 µM80August 19
Primer ccdB1127100100 µM27August 19
Primer ccdB1128100100 µM29August 19
Primer K1451501129100100 µM31August 19
Primer K1451501130100100 µM34August 19
Primer ccdB11279020 µM28August 19
Primer ccdB112898020 µM30August 19
Primer K14515011299901 µM33August 19
Primer K14515011299020 µM32August 19
Primer K14515011309901 µM36August 19
Primer K14515011309020 µM35August 19
Primer K145014 (2.1)1156990100 µM37Sept 01
Primer K145014 (2.1)11569020 µM38Sept 01
Primer Suffix1153990100 µM39Sept 02
Primer Suffix11539020 µM40Sept 02
Primer Prefix1152990100 µM41Sept 02
Primer Prefix11529020 µM42Sept 02