Team:MIT/Parts

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Parts Submitted to the Registry

GFP

Although there are multiple GFP parts in the registry already, this part uses the modified Silver BioBrick prefix and suffix to allow for protein construction.

  • BioBrick Number:
  • Sequence:

p1025

This sequence codes for a short peptide, found to competitively inhibit binding of S.mutans to the tooth surface (Kelly CG et al.; Nature Biotechnol. 1999). S.mutans takes in sugars and secretes lactic acid, causing dental cavities, so introduction of this peptide into the mouth prevents colonies. This part uses the modified Silver BioBrick prefix and suffix to allow for protein construction.

  • BioBrick Number:
  • Sequence:

L.bulgaricus signal

This is a short signal sequence recognized as a secretion tag by Lactobacillus strains. It was isolated at the 5' end of the prtB gene in Lactobacillus bulgaricus, which codes for a lactose digesting protein that is bound to the surface ([http://www.ncbi.nlm.nih.gov/pubmed/12788739?dopt=Abstract| Germond JE et al.; Appl Environ Microbiol. 2003]).



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