Team:MIT/Project

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<!-- *** What falls between these lines is the Alert Box!  You can remove it from your pages once you have read and understood the alert *** -->
 
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<div id="template" style="text-align: center; font-weight: bold; font-size: large; color: #f6f6f6; padding: 5px;">
 
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This is a template page. READ THESE INSTRUCTIONS.
 
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<div id="instructions" style="text-align: center; font-weight: normal; font-size: small; color: #f6f6f6; padding: 5px;">
 
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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
 
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<div id="warning" style="text-align: center; font-weight: bold; font-size: small; color: #f6f6f6; padding: 5px;">
 
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You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, and a lab notebook.  PLEASE keep all of your pages within your Team:Example namespace. 
 
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{|align="justify"
 
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
 
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|[[Image:Example_logo.png|200px|right|frame]]
 
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''Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
 
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|[[Image:Team.png|right|frame|Your team picture]]
 
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|-
 
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|align="center"|[[Team:MIT | Team Example 2]]
 
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<!--- The Mission, Experiments --->
<!--- The Mission, Experiments --->
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
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{| style="color:#1b2c8a;background-color:#CCCCCC;" cellpadding="3" cellspacing="1" border="1" bordercolor="#green" width="62%" align="center"
!align="center"|[[Team:MIT|Home]]
!align="center"|[[Team:MIT|Home]]
!align="center"|[[Team:MIT/Team|The Team]]
!align="center"|[[Team:MIT/Team|The Team]]
!align="center"|[[Team:MIT/Project|The Project]]
!align="center"|[[Team:MIT/Project|The Project]]
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!align="center"|[[Team:MIT/Experiments|Experiments]]
!align="center"|[[Team:MIT/Parts|Parts Submitted to the Registry]]
!align="center"|[[Team:MIT/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:MIT/Modeling|Modeling]]
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!align="center"|[[Team:MIT/Modeling|Results]]
!align="center"|[[Team:MIT/Notebook|Notebook]]
!align="center"|[[Team:MIT/Notebook|Notebook]]
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(''Or you can choose different headings.  But you must have a team page, a project page, and a notebook page.'')
 
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== '''Overall project''' ==
== '''Overall project''' ==
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Your abstract
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A clinical study (Kelly CG et al.; Nature Biotechnol. 1999) reports that a short 20 amino acid synthetic peptide called p1025 can reduce oral colonization of a major tooth-decaying bacterium Streptococcus mutans and thus help to maintain oral health.  The peptide does so by competitively inhibit binding of S. mutans to a glycoprotein from saliva on tooth surface.  The peptide is considered advantageous because it selectively prevents S. mutans colonization without removing beneficial bacteria that are also present in the mouth. In fact, the removal of S.mutans allows other (unharmful) bacteria to colonize these regions. It was found that introduction of p1025 into the mouth can inhibit S.mutans colonization for up to about 90 days.
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Our research goal is to engineer one of the common yogurt bacteria, Lactobacillus bulgaricus, to secrets p1025 in yogurt. Consumption of this yogurt after a meal could replace teeth brushing and reduce tooth decay significantly (although it wouldn't help with bad breath!)
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We are not going to market this yogurt. However, our project will demonstrate a bio-engineering approach to increase the value of common probiotics. Engineering yogurt is especially exciting. A new batch of yogurt is made by taking a small sample of the previous batch and using that to provide the bacteria for the new batch. This means that a starting culture of engineered L.bulgaricus can be used to provide a continuous supply of teeth cleaning yogurt. Since the p1025 gene could be replaced by any other gene or sequence of genes, any number of useful yogurt strains could be made. We believe that this could be the key to necessary, sustainable, and effective health care in small-scale underdeveloped countries, especially if yogurt is already an integral part of the diet.
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== '''Project Details''' ==
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== Project Details==
 
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=== Part 2 ===
 
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=== Stage 1 ===
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*Construction of p1025 fusion peptide with appropriate markers and expression of gene in E. coli. This is an intermediate step to evaluate gene construction and protein efficacy
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===Stage 2===
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*Binding Assay - To reproduce the results of the Nature paper and test if the p1025 construct made in stage 1 inhibits binding of Streptococcus mutans to hydroxyapatite beads as well as the original p1025 peptide.
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===Stage 3===
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*Expression of p1025 in Lactobacillus bulgaricus
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===Stage 4===
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*Make yogurt with modified Lactobacillus and test for inhibition of ''S. mutants'' binding
=== The Experiments ===
=== The Experiments ===
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*For daily information about wet-lab experiments performed, please follow directions in the "Procedural Notebook section" of [[https://2008.igem.org/Team:MIT/Notebook]]
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*For any further details regarding experiments, please refer to the appropriate sub-sections of [[https://2008.igem.org/Team:MIT/Notebook]]
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=='''A Visual Representation'''==
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[[Image:tooth.png|center]]
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<!--- The Mission, Experiments --->
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=== Part 3 ===
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{| style="color:#1b2c8a;background-color:#CCCCCC;" cellpadding="3" cellspacing="1" border="1" bordercolor="#green" width="62%" align="center"
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!align="center"|[[Team:MIT|Home]]
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!align="center"|[[Team:MIT/Team|The Team]]
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!align="center"|[[Team:MIT/Project|The Project]]
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!align="center"|[[Team:MIT/Experiments|Experiments]]
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!align="center"|[[Team:MIT/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:MIT/Modeling|Modeling]]
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!align="center"|[[Team:MIT/Notebook|Notebook]]
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|}
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== Results ==
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__NOTOC__

Latest revision as of 04:00, 30 October 2008


Home The Team The Project Experiments Parts Submitted to the Registry Results Notebook


Overall project

A clinical study (Kelly CG et al.; Nature Biotechnol. 1999) reports that a short 20 amino acid synthetic peptide called p1025 can reduce oral colonization of a major tooth-decaying bacterium Streptococcus mutans and thus help to maintain oral health. The peptide does so by competitively inhibit binding of S. mutans to a glycoprotein from saliva on tooth surface. The peptide is considered advantageous because it selectively prevents S. mutans colonization without removing beneficial bacteria that are also present in the mouth. In fact, the removal of S.mutans allows other (unharmful) bacteria to colonize these regions. It was found that introduction of p1025 into the mouth can inhibit S.mutans colonization for up to about 90 days.

Our research goal is to engineer one of the common yogurt bacteria, Lactobacillus bulgaricus, to secrets p1025 in yogurt. Consumption of this yogurt after a meal could replace teeth brushing and reduce tooth decay significantly (although it wouldn't help with bad breath!)

We are not going to market this yogurt. However, our project will demonstrate a bio-engineering approach to increase the value of common probiotics. Engineering yogurt is especially exciting. A new batch of yogurt is made by taking a small sample of the previous batch and using that to provide the bacteria for the new batch. This means that a starting culture of engineered L.bulgaricus can be used to provide a continuous supply of teeth cleaning yogurt. Since the p1025 gene could be replaced by any other gene or sequence of genes, any number of useful yogurt strains could be made. We believe that this could be the key to necessary, sustainable, and effective health care in small-scale underdeveloped countries, especially if yogurt is already an integral part of the diet.

Project Details

Stage 1

  • Construction of p1025 fusion peptide with appropriate markers and expression of gene in E. coli. This is an intermediate step to evaluate gene construction and protein efficacy

Stage 2

  • Binding Assay - To reproduce the results of the Nature paper and test if the p1025 construct made in stage 1 inhibits binding of Streptococcus mutans to hydroxyapatite beads as well as the original p1025 peptide.

Stage 3

  • Expression of p1025 in Lactobacillus bulgaricus

Stage 4

  • Make yogurt with modified Lactobacillus and test for inhibition of S. mutants binding

The Experiments

  • For daily information about wet-lab experiments performed, please follow directions in the "Procedural Notebook section" of [[1]]
  • For any further details regarding experiments, please refer to the appropriate sub-sections of [[2]]


A Visual Representation

Tooth.png


Home The Team The Project Experiments Parts Submitted to the Registry Modeling Notebook


Retrieved from "http://2008.igem.org/Team:MIT/Project"