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Team:Mexico-UNAM-IPN/Horizontal-Transfer/9 September 2008
From 2008.igem.org
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#4µl of each overnight culture was taken with 5ml of LB. Incubation 3hrs at 37°C at 150rpm. Three controls were made and three with Tet. Each control corresponds to one strain. | #4µl of each overnight culture was taken with 5ml of LB. Incubation 3hrs at 37°C at 150rpm. Three controls were made and three with Tet. Each control corresponds to one strain. | ||
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#After 3 hours 7.5µl of the Tet stock were added to kill the bacteria population that should be in the exponential phase. | #After 3 hours 7.5µl of the Tet stock were added to kill the bacteria population that should be in the exponential phase. | ||
Latest revision as of 03:51, 28 October 2008
CONTINUES FROM THE SEPTEMBER 8 ENTRY
New antibiotic calculus
Ampicilin
- Stock 100mg/ml
- Lethal concentration 512µg/ml
- 5.12µl from the stock have to be added to the media.
Tetracycline
- Stock 5mg/ml
- Lethal concentration 7.5µg/ml
- 1.5µl from the stock have to be added to the media.
- 4µl of each overnight culture was taken with 5ml of LB. Incubation 3hrs at 37°C at 150rpm. Three controls were made and three with Tet. Each control corresponds to one strain.
- After 3 hours 7.5µl of the Tet stock were added to kill the bacteria population that should be in the exponential phase.
