Ligation Standard Protocol
Ligations are used to combine DNA fragments.
1. Generally, use 20 μL for total ligation volume
2. Use 10% ligase
3. Add the ligase buffer
4. Usually have between 2 and 6 μL of DNA in ligation mixture
5. Balance of ligation mixture should be deionized water
6. Allow ligation to set in 4 oC fridge or on desktop overnight