Team:Mississippi State/14 July 2008

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  1. Pick colony from plates and prepare 2ml LB culture + Amp
  2. grow @37C ~10hrs
  3. transfer 1.5ml to microtube, centrifuge 5 min.
  4. extract plasmid using QIAgen kit
  5. prepare and run 1% agarose gel
  6. prepare pGEM mix, refrigerate overnight
  7. grow overnight XLIblue culture
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