Team:Montreal

From 2008.igem.org

(Difference between revisions)

Latest revision as of 05:25, 6 August 2008

Home	The Team	The Project	Parts	Notebook	Modeling	Links	Sponsors

Project Overview: Synchronizing the Repressilator

Background

A sinusoidal wave demonstrates a circadian rhythm

Although typically used to describe physical phenomena, oscillations are also observed in a range of biological processes such as circadian rhythms, pacemaker cells, neuronal communication and nephron function. Elowitz and Leibler (2000) have designed a genetic system called the Repressilator, composed of three genes (λcI, TetR, LacI) tied in negative feedback loops, which generates oscillations in protein expression within Escherichia coli.

In a Nutshell

Unlike oscillating cells in the human body, the Repressilator does not allow for the synchronization of oscillations in a population of cells. We attempt to bring oscillating cells into phase with eachother by coupling via the "autoinducer" molecule AI. Our 2-gene construct may also be used in future genetic systems, making it a system of choice in a highly modular synthetic biology framework.

Relevance and inspiration

While it may be presumptuous to call the synchronization of Elowitz's Repressilator the proverbial 'Holy Grail' of modern bioengineering, parallels can be drawn between these two that tempt the leap to association. Synchronization is a crucial stepping stone towards the understanding of natural oscillatory systems and towards creating a synthetic system that can accurately emulate this natural phenomenon. Innumerable past, present and future bioengineering projects in iGEM and the scientific community focus and revolve around this concept and its applications. By exploring the fundamental theory through mathematical models and empirical observation, we hope to understand the nature of these systems and develop future synthetic applications. •

@@ Line 1: / Line 1: @@
+<center>[[Team:Montreal/Sponsors|Your logo here!]]</center>
 {| align="center"
 |align="center" |[[Image:mcgill front2.jpg|850 px]]
@@ Line 14: / Line 15: @@
-== '''Project Overview: Elucidating an Experimentally Viable Repressilator''' ==
+== '''Project Overview: Synchronizing the Repressilator''' ==
 ===Background===
-[[Image:mcgill_osc.jpg||thumb|left|300px | A sinusoidal wave demonstrates a circadian rhythm]]Although typically used to describe physical phenomena, oscillations are also observed in a range of biological processes such as the circadian rhythm, neuronal communication and nephron function. [http://www.aph.caltech.edu/people/elowitz_m.html Elowitz] and Leibler ([http://www.aph.caltech.edu/people/Repressilator.pdf 2000]) postulated that a relaxation oscillator system called the Repressilator, composed of three genes (&lambda;cI, TetR, LacI) tied in negative feedback loops, would generate oscillations in protein expression within ''Escherichia coli''. Using one of these genes (TetR) linked with a Yellow Fluorescent Protein (YFP) gene to create a reporter plasmid, we expect to see the cells blinking over time as the concentration of the relevant proteins in the cell fluctuate.
+[[Image:Osc-rep.jpg||thumb|right|300px | A sinusoidal wave demonstrates a circadian rhythm]]Although typically used to describe physical phenomena, oscillations are also observed in a range of biological processes such as circadian rhythms, pacemaker cells, neuronal communication and nephron function. [http://www.aph.caltech.edu/people/elowitz_m.html Elowitz] and Leibler ([http://www.aph.caltech.edu/people/Repressilator.pdf 2000]) have designed a genetic system called the Repressilator, composed of three genes (&lambda;cI, TetR, LacI) tied in negative feedback loops, which generates oscillations in protein expression within ''Escherichia coli''.
-<br><br><br>
-<br>
-<br>
+===In a Nutshell===
-==Relevance and inspiration==
+Unlike oscillating cells in the human body, the Repressilator does not allow for the synchronization of oscillations in a population of cells. We attempt to bring oscillating cells into phase with eachother by coupling via the "autoinducer" molecule AI. Our 2-gene construct may also be used in future genetic systems, making it a system of choice in a highly modular synthetic biology framework.
-While it may be presumptuous to call Elowitz's Repressilator the proverbial '[http://en.wikipedia.org/wiki/Holy_Grail Holy Grail]' of modern bioengineering, parallels can be drawn between these two that tempt the leap to association. The Repressilator, often theorized about but never reproduced in a laboratory, is a crucial stepping stone towards a true understanding of synthetic oscillatory systems and the road towards creating a synthetic system that emulate the natural. Innumerable past, present and future bioengineering projects in iGEM and the real scientific community focus and revolve around this concept and its applications. By exploring the fundamental theory through mathematical models and empirical observation, we hope to understand the nature of these systems and develop future synthetic applications.
+===Relevance and inspiration===
+While it may be presumptuous to call the synchronization of Elowitz's Repressilator the proverbial '[http://en.wikipedia.org/wiki/Holy_Grail Holy Grail]' of modern bioengineering, parallels can be drawn between these two that tempt the leap to association. Synchronization is a crucial stepping stone towards the understanding of natural oscillatory systems and towards creating a synthetic system that can accurately emulate this natural phenomenon. Innumerable past, present and future bioengineering projects in iGEM and the scientific community focus and revolve around this concept and its applications. By exploring the fundamental theory through mathematical models and empirical observation, we hope to understand the nature of these systems and develop future synthetic applications.
 &bull;
 [[Image:mcgillwinterpng.png|800px|center|McGill in winter]]