Team:Montreal/DNA Agarose Gel

From 2008.igem.org

Revision as of 23:38, 16 June 2008 by KenSun (Talk | contribs)
Home The Team The Project Parts Submitted to the Registry Modeling Notebook

Procedure

1. Measure 50mL of TAE buffer and mix with 0.5g of Agarose powder.

2. Thoroughly mix the two in the flask and microwave the mixture for roughly about 1 minute (or until you see bubbling).

3. Add 1.5µL of Ethidium Bromide and swirl until a fine mixture is seen.

4. Pour the mixture onto the casting tray (make sure that each end is taped), with a comb inserted in one of the ends.

5. Wait for the gel to solidify over time.

6. Once hardened, remove the comb and load the gel into the electrophoresis box.