http://2008.igem.org/wiki/index.php?title=Team:NTU-Singapore/Modelling/Results&feed=atom&action=historyTeam:NTU-Singapore/Modelling/Results - Revision history2024-03-28T15:07:21ZRevision history for this page on the wikiMediaWiki 1.16.5http://2008.igem.org/wiki/index.php?title=Team:NTU-Singapore/Modelling/Results&diff=10225&oldid=prevLalala8585: Removing all content from page2008-06-30T15:09:52Z<p>Removing all content from page</p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">=Results of Modeling=</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">==Lactose Induced GFP production==</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">[[Image:GFP_LACTOSE.jpg|850px| GFP Production Model]]</del></div></td><td colspan="2"> </td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">==E7 production system==</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">[[Image:Lactose.jpg|850px| E7 Production Model]]</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">[[Image:Varying_lactose_input.jpg|850px| E7 Production Modeling Results]]</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">Modeling of the System shows that Lactose induction is essential to produce E7 and a variation of Lactose input can result in different yields of E7</del></div></td><td colspan="2"> </td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">==Lysis Production system==</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">[[Image:Lysis.jpg|850px| Lysis Production Model]]</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">[[Image:Varying_Lysis.jpg|850px|Lysis Prodcution Modeling Results]]</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">Here we define 1 as a certain threshold e.g. (<250 µM) that when the lysis protein reaches it, lysis in the cell definitely occurs.</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">Although we wish for an absolute AND gate, where 0 will have no lysis production at all, simulation on biological systems shows that such results are impossible. Both addition Fe ions and Ai-2 alone would induce a certain level of lysis production. However when both are available, the lysis protein production would be higher. </del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">In the presence of Ai-2 alone, Lysis will most likely still occur albeit at a slower rate compared to situation when both Fe and Ai-2 are present.</del></div></td><td colspan="2"> </td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">With the addition of iron and Ai-2 together the rate of lysis production is still significantly much higher compared to Ai-2 alone.</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">Therefore, by giving the logical output ‘1’ as a suitable threshold value of Lysis protein production higher than that of Ai-2 induction alone, we would still be able to obtain an AND gate based on our definition.</del></div></td><td colspan="2"> </td></tr>
</table>Lalala8585http://2008.igem.org/wiki/index.php?title=Team:NTU-Singapore/Modelling/Results&diff=9110&oldid=prevLalala8585 at 04:15, 26 June 20082008-06-26T04:15:50Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=Results of Modeling=</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=Results of Modeling=</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">==Lactose Induced GFP production==</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[Image:GFP_LACTOSE.jpg|850px| GFP Production Model]]</ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==E7 production system==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==E7 production system==</div></td></tr>
</table>Lalala8585http://2008.igem.org/wiki/index.php?title=Team:NTU-Singapore/Modelling/Results&diff=9108&oldid=prevLalala8585 at 04:02, 26 June 20082008-06-26T04:02:34Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==E7 production system==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==E7 production system==</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[Image:Lactose.jpg|850px| E7 Production Model]]</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:Varying_lactose_input.jpg|850px| E7 Production Modeling Results]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:Varying_lactose_input.jpg|850px| E7 Production Modeling Results]]</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Lysis Production system==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Lysis Production system==</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[Image:Lysis.jpg|850px| Lysis Production Model]]</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:Varying_Lysis.jpg|850px|Lysis Prodcution Modeling Results]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:Varying_Lysis.jpg|850px|Lysis Prodcution Modeling Results]]</div></td></tr>
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</table>Lalala8585http://2008.igem.org/wiki/index.php?title=Team:NTU-Singapore/Modelling/Results&diff=9103&oldid=prevLalala8585: New page: <html><link rel="stylesheet" href="http://greenbear88.googlepages.com/ntu_igem.css" type="text/css"></html> <div id="header">{{User:Greenbear/sandbox/header}}</div> <div id="maincontent"...2008-06-26T03:55:24Z<p>New page: <html><link rel="stylesheet" href="http://greenbear88.googlepages.com/ntu_igem.css" type="text/css"></html> <div id="header">{{User:Greenbear/sandbox/header}}</div> <div id="maincontent"...</p>
<p><b>New page</b></p><div><html><link rel="stylesheet" href="http://greenbear88.googlepages.com/ntu_igem.css" type="text/css"></html><br />
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=Results of Modeling=<br />
<br />
==E7 production system==<br />
[[Image:Varying_lactose_input.jpg|850px| E7 Production Modeling Results]]<br />
<br />
Modeling of the System shows that Lactose induction is essential to produce E7 and a variation of Lactose input can result in different yields of E7<br />
<br />
==Lysis Production system==<br />
[[Image:Varying_Lysis.jpg|850px|Lysis Prodcution Modeling Results]]<br />
<br />
Here we define 1 as a certain threshold e.g. (<250 µM) that when the lysis protein reaches it, lysis in the cell definitely occurs.<br />
Although we wish for an absolute AND gate, where 0 will have no lysis production at all, simulation on biological systems shows that such results are impossible. Both addition Fe ions and Ai-2 alone would induce a certain level of lysis production. However when both are available, the lysis protein production would be higher. <br />
<br />
<br />
In the presence of Ai-2 alone, Lysis will most likely still occur albeit at a slower rate compared to situation when both Fe and Ai-2 are present.<br />
<br />
With the addition of iron and Ai-2 together the rate of lysis production is still significantly much higher compared to Ai-2 alone.<br />
<br />
Therefore, by giving the logical output ‘1’ as a suitable threshold value of Lysis protein production higher than that of Ai-2 induction alone, we would still be able to obtain an AND gate based on our definition.</div>Lalala8585