NTU@iGEM Project Introduction Methodology Team Introduction Team background Notebook Dry Lab Introduction ODEs used Modeling Constructs Parameters used Deterministic Modeling Sensitivity Analysis Stochastic Modeling Wetlab Introduction Materials and Equipment Protocols Verification of Detection & Lysis System Our New Parts Characterization Introduction Standard Promoters Characterization of pLacI-GFP pLacI-GFP images and clips Characterization of pLsrA-YFP New characterization Proposal Parameter Estimation & Correlations Acknowledgements

Sensitivity Analysis

A sensitivty Analysis for the two systems were performed using [http://www.sbtoolbox2.org/main.php SBtoolbox 2]. The reason for performing this analysis is to find out which parameters are of high importance and would have a major effects on the experimental results. A part of the reason is because some of the paramters were fitted and not obtained strictly from literature. This analysis would allow us to plan for experiments while taking into account the effect these parameters may have.

Lactose Sensitivity Analysis

Lysis Sensitivity Analysis

The Sensitivity analysis revealed that the degradation rate of Proteins was a major factor and the previous assumption was not that accurate. The value for protein degradation was adjusted by assuming that the half time for protein degradation is 10 minutes. The new Lysis output according to the model is as follows.

As we can see, the output of protein has been drastically reduced from the previous modeling exercise for the Lysis Protein Production system.