Team:NTU-Singapore/Notebook/12 September 2008

From 2008.igem.org

Revision as of 02:40, 28 October 2008 by Lalala8585 (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Friday 12 September:

  • 1200am: punching and dilution into TE buffer at 50 degrees C of pSB2K3 (for obtaining plasmid vector with KanR).
  • 1230pm: digestion with E/P for Detection system (LsrA-RBS-Lysis-Term) (insert) and pSB2K3 (vector). Incubate for 2 hours until 3pm.
  • 2pm-530pm: prepare LBK plates.
  • 3pm-415pm: MinElute purification and Ligation (followed by another purification) for the insert and vector digested at 1230pm.
  • 4pm: punching for BBa_E0430 (RBS-YFP-Term) and BBa_E0420 (RBS-CFP-Term)
  • 430-6pm: Transformation for:
    • RBS-YFP-Term and RBS-YFP-Term into Top10 cells. (LBA plates)
    • LsrA-RBS-Lysis-Term (insert) ligated with pSB2K3 (vector) into top10 (and use LBK plate for selection).
    • Detection system (LsrA-RBS-Lysis-Term) and Production system (LacI-RBS-E7-Term) into LuxS k/o (LBA plates).
Retrieved from "http://2008.igem.org/Team:NTU-Singapore/Notebook/12_September_2008"