Team:NTU-Singapore/Notebook/25 July 2008

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=Friday 25 July=
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<div class="quote" style="margin:20px;">
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{|border="1" style="background-color:#ffffcc;" cellpadding="20"
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*Darius
*Darius
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**Miniprep 23 tubes of bacteria, E7-ter, lysis, lsrA respectively.
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**Miniprep 23 tubes of bacteria, E7-ter, Lysis Terminator, lysis, lsrA respectively.
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*Digestion of E7-terminator, Lysis Standard Vector, LsrA standard vector.
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*Digestion of E7-terminator, lysis Terminator, Lysis Standard Vector, LsrA standard vector.
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Heat inactivation at 80 celcius in thermal cycler before gel run.
*Run Gel Check
*Run Gel Check
**E7 showed a 2k Band, however, this is not conclusive as GFP vector and E7 Is of the same size.
**E7 showed a 2k Band, however, this is not conclusive as GFP vector and E7 Is of the same size.
Lysis standard vector is successful, showed a band between 100bp and 200bp(140bp)
Lysis standard vector is successful, showed a band between 100bp and 200bp(140bp)
LsrA standard vector failed.
LsrA standard vector failed.
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Lysis Terminator was kept in the fridge for saturday gel run due to time constraits i had on friday.
*PCR verification of E7 terminator. Ran PCR with different constituents.
*PCR verification of E7 terminator. Ran PCR with different constituents.
**Old E7 Primers, New E7 primers, E7 verification primers.
**Old E7 Primers, New E7 primers, E7 verification primers.
* Results seen on saturday morning.
* Results seen on saturday morning.
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Latest revision as of 02:33, 28 October 2008

Friday 25 July

  • Darius
    • Miniprep 23 tubes of bacteria, E7-ter, Lysis Terminator, lysis, lsrA respectively.
  • Digestion of E7-terminator, lysis Terminator, Lysis Standard Vector, LsrA standard vector.

Heat inactivation at 80 celcius in thermal cycler before gel run.

  • Run Gel Check
    • E7 showed a 2k Band, however, this is not conclusive as GFP vector and E7 Is of the same size.

Lysis standard vector is successful, showed a band between 100bp and 200bp(140bp) LsrA standard vector failed. Lysis Terminator was kept in the fridge for saturday gel run due to time constraits i had on friday.

  • PCR verification of E7 terminator. Ran PCR with different constituents.
    • Old E7 Primers, New E7 primers, E7 verification primers.
  • Results seen on saturday morning.
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