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Team:NTU-Singapore/Notebook/25 September 2008
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=Thursday 25 September= | =Thursday 25 September= | ||
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*Minipreps for LsrA-YFP, followed by digestional gel check. | *Minipreps for LsrA-YFP, followed by digestional gel check. | ||
**Result: correct bands: 1kb insert (LsrA-RBS with YFP generator); 2kb vector | **Result: correct bands: 1kb insert (LsrA-RBS with YFP generator); 2kb vector | ||
*Make LuxS mutants more competent. Then transform detection system and production system into these newly made LuxS mutants. | *Make LuxS mutants more competent. Then transform detection system and production system into these newly made LuxS mutants. | ||
*Grow W3110 in LB plus 0.8% glucose for 4 hours, centrifuge and take the supernatant (which contains AI-2), store at -20 degrees C. | *Grow W3110 in LB plus 0.8% glucose for 4 hours, centrifuge and take the supernatant (which contains AI-2), store at -20 degrees C. | ||
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Revision as of 13:11, 25 October 2008
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Thursday 25 September
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