Team:NTU-Singapore/Notebook/27 July 2008

From 2008.igem.org

(Difference between revisions)
 
Line 3: Line 3:
<div id="header">{{User:Greenbear/sandbox/header}}</div>
<div id="header">{{User:Greenbear/sandbox/header}}</div>
-
<div id="maincontent" style="margin-top:100px;">
+
<div id="maincontent" style="margin-top:130px;">
<html>
<html>
<div id="arrow">
<div id="arrow">

Latest revision as of 02:34, 28 October 2008

Sunday 27 July

Darius

  • Digested Lysis and terminator for an 1 and a half hour.
    • did a variation of lysis and terminator between inserts and vectors.
      • Gel extracted lysis insert(E & X)(sequential digestion, PCR purification)and lysis vector(E and S)( Non sequential). Gel extracted terminator insert(S & P) and terminator vector(E & X) (sequential).
  • Fast ligation.
  • Transformation