Team:NTU-Singapore/Notebook/29 August 2008

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Latest revision as of 02:38, 28 October 2008


Friday 29 August

  • Lu Chao: prepared new top10 competent cells (60x50ul tubes).
  • Hung: transformation into top10 for:
    • The remaining ligation mixture of LacI-RBS-E7-Term.
    • LacI-RBS (as only 1 tube of LacI-RBS was left)(for minipreps purpose).Also, newly prepared top10 was used to transform as a check of their efficiency.
    • LsrA-RBS-Lysis-Term (our detection system that was done on Tuesday). (for minipreps purpose).Also, newly prepared top10 was used to transform as a check of their efficiency.
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