Lab details:

Personnel

Choon Kit, Hung, Darius:

• Morning:
  • Take out 4 cryotubes (2 terminators, 2 LacI-GFP) from yesterday incubation, then spin them down, pour out the liquid to obtain cell pellets.
  • Prepare 6 digestion solutions for gel electrophoresis in the afternoon:
    1. 2 for terminators
    2. 2 for LacI-GFP
    3. 1 for CoE7 plasmid from BW strain (from -20 oC fridge)
    4. 1 for CoE7 plasmid from W3110 strain (from -20 oC fridge)

• Afternoon:
  • Amplification for the following plasmids (from Registry):
    1. GFP generator [http://partsregistry.org/wiki/index.php?title=Part:BBa_E0240 BBa_E0240]
    2. RBS [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0032 BBa_B0032] and RBS [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034]
    3. T7 promoter [http://partsregistry.org/wiki/index.php?title=Part:BBa_I719005 BBa_I719005]
  • Run gel for morning samples.
  • Inoculation of BW (2x),W3110 (2x), MG1655 (2x) for glycerol stock on Thursday.
  • Prepare LB broth
  • Prepare 17x LBA agar plates