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| - | <!-- *** What falls between these lines is the Alert Box! You can remove it from your pages once you have read and understood the alert *** --> | + | <html><link rel="stylesheet" href="http://edventure.ntu.edu.sg/bbcswebdav/users/z060022/ntu_igem.css" type="text/css"></html> |
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| - | <html>
| + | <div id="header">{{User:Greenbear/sandbox/header}}</div> |
| - | <div id="box" style="width: 700px; margin-left: 137px; padding: 5px; border: 3px solid #000; background-color: #fe2b33;"> | + | |
| - | <div id="template" style="text-align: center; font-weight: bold; font-size: large; color: #f6f6f6; padding: 5px;">
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| - | This is a template page. READ THESE INSTRUCTIONS.
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| - | </div>
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| - | <div id="instructions" style="text-align: center; font-weight: normal; font-size: small; color: #f6f6f6; padding: 5px;">
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| - | You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
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| - | </div> | + | |
| - | <div id="warning" style="text-align: center; font-weight: bold; font-size: small; color: #f6f6f6; padding: 5px;">
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| - | You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, and a lab notebook. PLEASE keep all of your pages within your Team:Example namespace.
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| - | </div>
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| - | </div>
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| - | </html>
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| - | <!-- *** End of the alert box *** --> | + | <div id="body" style="margin-top:200px;width:950px;padding:5px;"> |
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| | + | ==Characterization of Parts== |
| | + | We are interested in characterizing the parts |
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| | + | i) Lactose/IPTG sensitive Promoter pLac [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0010 BBa_R0010] |
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| - | {|align="justify"
| + | ii) Iron Sensitive Promoter pFe [http://partsregistry.org/wiki/index.php?title=Part:BBa_I716014 BBa_I716014] |
| - | |You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.
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| - | |[[Image:Example_logo.png|200px|right|frame]]
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| - | |-
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| - | ''Tell us more about your project. Give us background. Use this is the abstract of your project. Be descriptive but concise (1-2 paragraphs)''
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| - | |[[Image:Team.png|right|frame|Your team picture]]
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| - | |align="center"|[[Team:NTU-Singapore | Team Example 2]]
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| - | |}
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| - | <!--- The Mission, Experiments --->
| + | The characterization is carried out by ligating the desired promoter with the Green Fluorescence protein: [http://partsregistry.org/Part:BBa_E0240 BBa_E02040] |
| | + | *For the pLac BBa_R0010, it is already available in the form we desired in the registry as [http://partsregistry.org/wiki/index.php?title=Part:BBa_J04430 BBa_J04430] |
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| - | {| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
| + | ==The FLx800™ Fluorescence Microplate Reader== |
| - | !align="center"|[[Team:NTU-Singapore|Home]]
| + | [[image: Ntu@iGEM_FLx_800_pic.jpg]] |
| - | !align="center"|[[Team:NTU-Singapore/Team|The Team]]
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| - | !align="center"|[[Team:NTU-Singapore/Project|The Project]]
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| - | !align="center"|[[Team:NTU-Singapore/Parts|Parts Submitted to the Registry]]
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| - | !align="center"|[[Team:NTU-Singapore/Modeling|Modeling]]
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| - | !align="center"|[[Team:NTU-Singapore/Notebook|Notebook]]
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| - | |}
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| - | (''Or you can choose different headings. But you must have a team page, a project page, and a notebook page.'')
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| - | | + | |
| - | | + | |
| - | ===Note===
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| - | | + | |
| - | If you choose to include a '''Parts Submitted to the Registry''' page, please list your parts here. This is not necessary but it may be a nice list to keep track of.
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Characterization of Parts
We are interested in characterizing the parts
i) Lactose/IPTG sensitive Promoter pLac BBa_R0010
ii) Iron Sensitive Promoter pFe BBa_I716014
The characterization is carried out by ligating the desired promoter with the Green Fluorescence protein: BBa_E02040
- For the pLac BBa_R0010, it is already available in the form we desired in the registry as BBa_J04430
The FLx800™ Fluorescence Microplate Reader
"
