Team:NTU-Singapore/Parts
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Introduction
The group proposed to discuss and investigate the working range of the parts and devices of our system
- The proposed variables to investigate are:
i) Concentration of activator
ii) Time
iii) Temperature
Characterization of Parts
We are interested in characterizing the parts
i) Lactose/IPTG sensitive Promoter pLac BBa_R0010
ii) Iron Sensitive Promoter pFe BBa_I716014
iii) plsrA-YFP
The characterization is carried out by ligating the desired promoter with the Green Fluorescence protein: BBa_E02040
- For the pLac BBa_R0010, it is already available in the form we desired in the registry as BBa_J04430
The FLx800™ Fluorescence Microplate Reader
The FLx800™ Fluorescence Microplate Reader was available for our use courtesy of Division of Bio-engineering lab. It was purchased from BioTek and for more information about the reader, you can refer to The FLx800™ Fluorescence Microplate Reader
The FLx800™ Fluorescence Microplate Reader has a computer software KCJunior that was installed in a computer adjacent to the reader for collection and processing of data from our samples.