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Team:Newcastle University/Protocols
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[[Making Frozen Glycerol Cell Stocks]] | [[Making Frozen Glycerol Cell Stocks]] | ||
Revision as of 14:15, 8 September 2008
Newcastle University
GOLD MEDAL WINNER 2008
| Home | Team | Original Aims | Software | Modelling | Proof of Concept Brick | Wet Lab | Conclusions |
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Isolating Plasmid from Cells (Miniprep)
Restricting Plasmids (Double Restriction)
Transformation Following Ligation
Making Frozen Glycerol Cell Stocks
Making Frozen Glycerol Cell Stocks
- Pippette 2μl overnight culture into an Eppendorf tube.
- Add 0.5ml glycerol and vortex briefly to mix.
- Prepare a freeze box by decanting a small amount of liquid nitrogen into the box. (N.B: wear protective gloves and face mask.)
- Place the tube in the box and keep at -80°C.
