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	[[Making Frozen Glycerol Cell Stocks]]		[[Making Frozen Glycerol Cell Stocks]]
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-	===Making Frozen Glycerol Cell Stocks===
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-	* Pippette 2μl overnight culture into an Eppendorf tube.
-	* Add 0.5ml glycerol and vortex briefly to mix.
-	* Prepare a freeze box by decanting a small amount of liquid nitrogen into the box. (N.B: wear protective gloves and face mask.)
-	* Place the tube in the box and keep at -80°C.

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Revision as of 14:15, 8 September 2008

Newcastle University

GOLD MEDAL WINNER 2008

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Making Frozen Glycerol Cell Stocks

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