Team:Newcastle University/Protocols

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Newcastle University

GOLD MEDAL WINNER 2008

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Protocols

Agarose Gel Electrophoresis

Making Agar Plates

Isolating Plasmid from Cells (Miniprep)

Restricting Plasmids (Double Restriction)

Purifying DNA from an enzymatic reaction

Cutting a Specific Band from Agarose Gel

Purifying DNA from Gel Slices

Transforming into DH5α or TOP10 E. coli

Making Frozen Glycerol Cell Stocks From Overnight Cultures

Making Overnight Cultures from Frozen Glycerol Cell Stocks

Making Overnight Cultures from Agar Colonies

Transforming into Bacillus subtillis

Inducing Bacillus subtilis with Subtilin

Preparing Bacillus subtilis for Microscopy

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