Team:Paris/August 13
From 2008.igem.org
(Difference between revisions)
(→Assay to purify a PCR product using the Qiaquick Gel Extraction kit) |
(→Assay to purify a PCR product using the Qiaquick Gel Extraction kit) |
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*30 µL of purified product + 12 µL of loading blue | *30 µL of purified product + 12 µL of loading blue | ||
*electrophoresis, 10 µL loaded | *electrophoresis, 10 µL loaded | ||
- | => see Gel 1, well | + | => see Gel 1, well n° 8 |
- | + | ||
==> results: PCR products can be purified using the Qiaquick Gel Extraction kit. We just have to replace the buffer PBI by the buffer QG! | ==> results: PCR products can be purified using the Qiaquick Gel Extraction kit. We just have to replace the buffer PBI by the buffer QG! | ||
Revision as of 12:41, 14 August 2008
Sequencing Minipreps we did yesterday
Minipreps: Plasmid extraction
Glycerol Stocks
Assay to purify a PCR product using the Qiaquick Gel Extraction kit
=> see Gel 1, well n° 8 ==> results: PCR products can be purified using the Qiaquick Gel Extraction kit. We just have to replace the buffer PBI by the buffer QG! PCR screening4 more transformants of L130 (pFlhB into J61002) are screened by PCR.
Electrophoresis
==> The L130 transformants analysed are not correct. Promoter Characterization: work plan |