Team:Paris/August 13
From 2008.igem.org
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4 more transformants of L130 (pFlhB into J61002) are screened by PCR. | 4 more transformants of L130 (pFlhB into J61002) are screened by PCR. | ||
*PCR screening programm | *PCR screening programm | ||
- | *elogation time : 1 min 30 | + | *elogation time: 1 min 30 |
*template: colonies from the transformation plate | *template: colonies from the transformation plate | ||
*positive control: S142 (J61002) | *positive control: S142 (J61002) |
Revision as of 12:43, 14 August 2008
Sequencing Minipreps we did yesterday
Minipreps: Plasmid extraction
Glycerol Stocks
Assay to purify a PCR product using the Qiaquick Gel Extraction kit
=> see Gel 1, well n° 8 ==> results: PCR products can be purified using the Qiaquick Gel Extraction kit. We just have to replace the buffer PBI by the buffer QG! PCR screening4 more transformants of L130 (pFlhB into J61002) are screened by PCR.
Electrophoresis
==> The L130 transformants analysed are not correct. Promoter Characterization: work plan |