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Team:Paris/August 5
From 2008.igem.org
Electrophoresis Purification of PCR
When the PCR cycles were finished,
- 10 µL of 6X loading dye were added
- The samples were then loaded (2 x 30 µL per sample) on a 1,5% agarose gel.
After electrophoresis, the bands corresponding to the right amplification were excised and purified using the QIAquick DNA Gel Extraction Kit (QIAGEN). The elution was made in 50 µL of water. Because the intensity of the band corresponding to MP 120 was very low, we only continued with MP 100. MP 100 was digested by EcoRI & SpeI (Forward Insert) or by XbaI & PstI (D100 : Backward Insert).
| Name | Promotor | Gel | Band | Expected size | Measured size |
| PCR_124 | pFlgA | ||||
| PCR_125 | pFlgB | ||||
| PCR_126 | pFlhB | ||||
| PCR_127 | pFlhDC |
==> Remark :
