Team:Paris/Construction

From 2008.igem.org

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| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(RBS WT)-ECFP tripart (LVA+)
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(RBS WT)-ECFP tripart (LVA+)
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|align="center"| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFLiL is ECFP.
|align="center"| [[Team:Paris/October 24|October 24]]
|align="center"| [[Team:Paris/October 24|October 24]]
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Revision as of 02:39, 30 October 2008

FIFO
Construction Description Validation Date
Icon regulatory.pngIcon rbs.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
pFliL(RBS WT)-ECFP tripart (LVA+)
Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFLiL is ECFP. October 24
Icon regulatory.pngIcon rbs.pngIcon reporter.png
pFliL(+ RBS WT)-GFP
October 24
Icon regulatory.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
pFlgA(+ RBS WT) - YFP Tripart (LVA+)
August 22
Icon regulatory.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
pFlgA(+ RBS WT) - YFP Tripart (LVA-)
August 22
Icon regulatory.pngIcon rbs.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
pFlgA(+ RBS WT)-YFP tripart (AAV+)
October 24
Icon regulatory.pngIcon rbs.pngIcon reporter.png
pFlgA(+ RBS WT)-GFP
October 24
Icon regulatory.pngIcon rbs.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
pFlhB(+ RBS WT)-mRFP tripart (+LVA)
October 24
Icon regulatory.pngIcon rbs.pngIcon reporter.png
pFlhB(+ RBS WT)-GFP
October 26
OSCILLATION
Icon regulatory.pngIcon rbs.pngIcon coding.png
pFliL(+ RBS WT)-EnvZ*
October 26
Icon regulatory.pngPart icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
pFliL(+ RBS WT)-EnvZ*-GFP generator
October 29
Icon regulatory.pngIcon rbs.pngIcon coding.png
pFlhB(+ RBS WT)-EnvZ*
October 24
Icon regulatory.pngPart icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
pFlhB(+ RBS WT)-EnvZ*-GFP generator
October 29
Icon regulatory.pngIcon coding.png
araC/pBad-EnvZ*
October 27
SYNCHRONISATION
Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
pLas - gfp Tripart
August 1
Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
pLas - ECFP
August 1
Icon regulatory.pngIcon rbs.pngIcon coding.pngPart icon terminator.pngPart icon terminator.png
strongest promoter-rbs-lasR-dble ter
August 20
Icon regulatory.pngIcon rbs.pngIcon coding.pngPart icon terminator.pngPart icon terminator.png
Strong promoter-rbs-lasR-dble ter
August 20
Part icon rbs.pngIcon coding.png
Strongest RBS (1)- LasR activator (+LVA)
August 13
Icon rbs.pngIcon coding.pngPart icon terminator.pngPart icon terminator.png
rbs-lasR + dbl ter
August 19
Icon coding.pngPart icon terminator.pngPart icon terminator.png
lasR activator + LVA - Double Terminator
August 1
Part icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
RBS lasI - GFP tripart
August 13
Part icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
RBS-lasI - ECFP
August 13
NEW GENE AND PROMOTER BIOBRICKS
Part icon regulatory.png
pFliA
October 04
Part icon regulatory.png
pFliL (+RBS WT)
October 10
Part icon regulatory.png
pFlgA (+RBS WT)
October 10
Part icon regulatory.png
pFlgB (+RBS WT)
October 10
Part icon regulatory.png
pFlhB (+RBS WT)
October 10
Icon coding.png
EnvZ*
September 04
Icon coding.png
FliA
September 04
Icon coding.png
FlhDC
August 13