Team:Paris/Construction

From 2008.igem.org

(Difference between revisions)
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| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFliL(+ RBS WT)-EnvZ*
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFliL(+ RBS WT)-EnvZ*
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|align=|  
+
|align=| pFliL is regulated by FlhDC and EnvZ regulates pFlhDC thanks to a negative feedback loop, origin of an oscillation behavior.
|align="center"| [[Team:Paris/October 26|October 26]]
|align="center"| [[Team:Paris/October 26|October 26]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(+  RBS WT)-EnvZ*-GFP generator
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFliL(+  RBS WT)-EnvZ*-GFP generator
-
|align=|  
+
|align=| The same construction as before but with a GFP in order to follow the dynamic of expression of pFliL.
|align="center"| [[Team:Paris/October 29|October 29]]
|align="center"| [[Team:Paris/October 29|October 29]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFlhB(+ RBS WT)-EnvZ*
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]]<br>pFlhB(+ RBS WT)-EnvZ*
-
|align=|  
+
|align=| pFlhB is regulated by FlhDC and EnvZ regulates pFlhDC thanks to a negative feedback loop, origin of an oscillation behavior.
|align="center"| [[Team:Paris/October 24|October 24]]
|align="center"| [[Team:Paris/October 24|October 24]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(+ RBS WT)-EnvZ*-GFP generator
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pFlhB(+ RBS WT)-EnvZ*-GFP generator
-
|align=|  
+
|align=| The same construction as before but with a GFP in order to follow the dynamic of expression of pFlhB.
|align="center"| [[Team:Paris/October 29|October 29]]
|align="center"| [[Team:Paris/October 29|October 29]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_coding.png]]<br>araC/pBad-EnvZ*
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_coding.png]]<br>araC/pBad-EnvZ*
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|align=|  
+
|align=| A construction made for characterizing the repression power of the mutated EnvZ on our system
|align="center"| [[Team:Paris/October 27|October 27]]
|align="center"| [[Team:Paris/October 27|October 27]]
|-
|-
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|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pLas - gfp Tripart
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pLas - gfp Tripart
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|align=|  
+
|align=| Construction for characterization of the pLas promoter
|align="center"| [[Team:Paris/August 1|August 1]]
|align="center"| [[Team:Paris/August 1|August 1]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pLas - ECFP
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>pLas - ECFP
-
|align=|  
+
|align=| The same as before but with another fluorophore
|align="center"| [[Team:Paris/August 1|August 1]]
|align="center"| [[Team:Paris/August 1|August 1]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> strongest promoter-rbs-lasR-dble ter
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> strongest promoter-rbs-lasR-dble ter
-
|align=|  
+
|align=| A construction for a strong constitutive expression of the Las Regulator in order to get maximal expression in presence of AHL
|align="center"| [[Team:Paris/August 20|August 20]]
|align="center"| [[Team:Paris/August 20|August 20]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> Strong promoter-rbs-lasR-dble ter
| style="background: #D4E2EF;" | [[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br> Strong promoter-rbs-lasR-dble ter
-
|align=|  
+
|align=| A derived construction of the previous  one for smaller constitutive expression of LasR in the Bacteria.
|align="center"| [[Team:Paris/August 20|August 20]]
|align="center"| [[Team:Paris/August 20|August 20]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]<br>Strongest RBS (1)- LasR activator (+LVA)
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]<br>Strongest RBS (1)- LasR activator (+LVA)
-
|align=|  
+
|align=| Intermediary construction. We uses different rbs for Las R in order to get various response dynamic to the presence of AHL
|align="center"| [[Team:Paris/August 13|August 13]]
|align="center"| [[Team:Paris/August 13|August 13]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>rbs-lasR + dbl ter
| style="background: #D4E2EF;" | [[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>rbs-lasR + dbl ter
-
|align=|  
+
|align=| Intermediary construction. We uses different rbs for Las R in order to get various response dynamic to the presence of AHL
|align="center"| [[Team:Paris/August 19|August 19]]
|align="center"| [[Team:Paris/August 19|August 19]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>lasR activator + LVA - Double Terminator
| style="background: #D4E2EF;" | [[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>lasR activator + LVA - Double Terminator
-
|align=|  
+
|align=| Intermediary construction
|align="center"| [[Team:Paris/August 1|August 1]]
|align="center"| [[Team:Paris/August 1|August 1]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS lasI - GFP tripart
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS lasI - GFP tripart
-
|align=|  
+
|align=| Construction that has to be associated with the FIFO. We will know when LasI is expressed and then when AHL is produced.
|align="center"| [[Team:Paris/August 13|August 13]]
|align="center"| [[Team:Paris/August 13|August 13]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS-lasI - ECFP  
| style="background: #D4E2EF;" | [[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]<br>RBS-lasI - ECFP  
-
|align=|  
+
|align=| The same as the previous one but with another fluorophore.
|align="center"| [[Team:Paris/August 13|August 13]]
|align="center"| [[Team:Paris/August 13|August 13]]
|-
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|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFliA
| style="background: #D4E2EF;" | [[Image:Part_icon_regulatory.png]]<br>pFliA
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|align=|  
+
|align=| Natural promoter of E. coli, extracted by PCR and then inserted in a biobrick vector. pFiA is regulated by FlhDC.
|align="center"| [[Team:Paris/October 04|October 04]]
|align="center"| [[Team:Paris/October 04|October 04]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   

Revision as of 03:05, 30 October 2008

FIFO
Construction Description Validation Date
Icon regulatory.pngIcon rbs.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
pFliL(RBS WT)-ECFP tripart (LVA+) 		Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFliL is ECFP fused with a LVA tag. October 24
Icon regulatory.pngIcon rbs.pngIcon reporter.png
pFliL(+ RBS WT)-GFP 		Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFliL is GFP. October 24
Icon regulatory.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
pFlgA(+ RBS WT) - YFP Tripart (LVA+) 		Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is EYFP fused with a LVA tag. August 22
Icon regulatory.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
pFlgA(+ RBS WT) - YFP Tripart (LVA-) 		Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is EYFP fused without a LVA tag. August 22
Icon regulatory.pngIcon rbs.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
pFlgA(+ RBS WT)-YFP tripart (AAV+) 		Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is EYFP fused with a AAV tag. October 24
Icon regulatory.pngIcon rbs.pngIcon reporter.png
pFlgA(+ RBS WT)-GFP 		Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is GFP. October 24
Icon regulatory.pngIcon rbs.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
pFlhB(+ RBS WT)-mRFP tripart (+LVA) 		Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlhB is mRFP with a LVA tag. October 24
Icon regulatory.pngIcon rbs.pngIcon reporter.png
pFlhB(+ RBS WT)-GFP 		Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlhB is GFP. October 26
OSCILLATION
Icon regulatory.pngIcon rbs.pngIcon coding.png
pFliL(+ RBS WT)-EnvZ* 		pFliL is regulated by FlhDC and EnvZ regulates pFlhDC thanks to a negative feedback loop, origin of an oscillation behavior. October 26
Icon regulatory.pngPart icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
pFliL(+ RBS WT)-EnvZ*-GFP generator 		The same construction as before but with a GFP in order to follow the dynamic of expression of pFliL. October 29
Icon regulatory.pngIcon rbs.pngIcon coding.png
pFlhB(+ RBS WT)-EnvZ* 		pFlhB is regulated by FlhDC and EnvZ regulates pFlhDC thanks to a negative feedback loop, origin of an oscillation behavior. October 24
Icon regulatory.pngPart icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
pFlhB(+ RBS WT)-EnvZ*-GFP generator 		The same construction as before but with a GFP in order to follow the dynamic of expression of pFlhB. October 29
Icon regulatory.pngIcon coding.png
araC/pBad-EnvZ* 		A construction made for characterizing the repression power of the mutated EnvZ on our system October 27
SYNCHRONISATION
Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
pLas - gfp Tripart 		Construction for characterization of the pLas promoter August 1
Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
pLas - ECFP 		The same as before but with another fluorophore August 1
Icon regulatory.pngIcon rbs.pngIcon coding.pngPart icon terminator.pngPart icon terminator.png
strongest promoter-rbs-lasR-dble ter 		A construction for a strong constitutive expression of the Las Regulator in order to get maximal expression in presence of AHL August 20
Icon regulatory.pngIcon rbs.pngIcon coding.pngPart icon terminator.pngPart icon terminator.png
Strong promoter-rbs-lasR-dble ter 		A derived construction of the previous one for smaller constitutive expression of LasR in the Bacteria. August 20
Part icon rbs.pngIcon coding.png
Strongest RBS (1)- LasR activator (+LVA) 		Intermediary construction. We uses different rbs for Las R in order to get various response dynamic to the presence of AHL August 13
Icon rbs.pngIcon coding.pngPart icon terminator.pngPart icon terminator.png
rbs-lasR + dbl ter 		Intermediary construction. We uses different rbs for Las R in order to get various response dynamic to the presence of AHL August 19
Icon coding.pngPart icon terminator.pngPart icon terminator.png
lasR activator + LVA - Double Terminator 		Intermediary construction August 1
Part icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
RBS lasI - GFP tripart 		Construction that has to be associated with the FIFO. We will know when LasI is expressed and then when AHL is produced. August 13
Part icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
RBS-lasI - ECFP 		The same as the previous one but with another fluorophore. August 13
NEW GENE AND PROMOTER BIOBRICKS
Part icon regulatory.png
pFliA 		Natural promoter of E. coli, extracted by PCR and then inserted in a biobrick vector. pFiA is regulated by FlhDC. October 04
Part icon regulatory.png
pFliL (+RBS WT) 		October 10
Part icon regulatory.png
pFlgA (+RBS WT) 		October 10
Part icon regulatory.png
pFlgB (+RBS WT) 		October 10
Part icon regulatory.png
pFlhB (+RBS WT) 		October 10
Icon coding.png
EnvZ* 		September 04
Icon coding.png
FliA 		September 04
Icon coding.png
FlhDC 		August 13
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