Team:Paris/Construction

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FIFO
Construction Description Validation Date
Icon regulatory.pngIcon rbs.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
pFliL(RBS WT)-ECFP tripart (LVA+)
Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFliL is ECFP fused with a LVA tag. October 24
Icon regulatory.pngIcon rbs.pngIcon reporter.png
pFliL(+ RBS WT)-GFP
Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFliL is GFP. October 24
Icon regulatory.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
pFlgA(+ RBS WT) - YFP Tripart (LVA+)
Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is EYFP fused with a LVA tag. August 22
Icon regulatory.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
pFlgA(+ RBS WT) - YFP Tripart (LVA-)
Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is EYFP fused without a LVA tag. August 22
Icon regulatory.pngIcon rbs.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
pFlgA(+ RBS WT)-YFP tripart (AAV+)
Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is EYFP fused with a AAV tag. October 24
Icon regulatory.pngIcon rbs.pngIcon reporter.png
pFlgA(+ RBS WT)-GFP
Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is GFP. October 24
Icon regulatory.pngIcon rbs.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
pFlhB(+ RBS WT)-mRFP tripart (+LVA)
Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlhB is mRFP with a LVA tag. October 24
Icon regulatory.pngIcon rbs.pngIcon reporter.png
pFlhB(+ RBS WT)-GFP
Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlhB is GFP. October 26
OSCILLATION
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pFliL(+ RBS WT)-EnvZ*
October 26
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pFliL(+ RBS WT)-EnvZ*-GFP generator
October 29
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pFlhB(+ RBS WT)-EnvZ*
October 24
Icon regulatory.pngPart icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
pFlhB(+ RBS WT)-EnvZ*-GFP generator
October 29
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araC/pBad-EnvZ*
October 27
SYNCHRONISATION
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pLas - gfp Tripart
August 1
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pLas - ECFP
August 1
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strongest promoter-rbs-lasR-dble ter
August 20
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Strong promoter-rbs-lasR-dble ter
August 20
Part icon rbs.pngIcon coding.png
Strongest RBS (1)- LasR activator (+LVA)
August 13
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rbs-lasR + dbl ter
August 19
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lasR activator + LVA - Double Terminator
August 1
Part icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
RBS lasI - GFP tripart
August 13
Part icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
RBS-lasI - ECFP
August 13
NEW GENE AND PROMOTER BIOBRICKS
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pFliA
October 04
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pFliL (+RBS WT)
October 10
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pFlgA (+RBS WT)
October 10
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pFlgB (+RBS WT)
October 10
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pFlhB (+RBS WT)
October 10
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EnvZ*
September 04
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FliA
September 04
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FlhDC
August 13