Team:Paris/July 14

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Results of the transformation :

Positive control : puc19 (1µl; 10pg/ml) --> No transformation
Biobricks 2007 : --> succeed of the transformation unlike the delay added in the ice
Biobricks 2008 : --> No transformation

          Conclusion : Cells are competents, because there is positive transformation
          Hypothesis : problem with the protocol of DNA extraction from the registery 2008

Culture of Cells :

Culture of DH5ɑ in 5ml of LB without Antibiotics

Preparation of medium and solutions :

1L of CCMB80
500ml of SOB ( 2 x 250ml)
500ml of SOC (2 x 250ml)
100ml of MgCl₂ (1M)
150ml of CaCl₂ (1M)
200ml of KOAC (1M)