"
Team:Paris/July 27
From 2008.igem.org
(Difference between revisions)
(→Results of extraction : Electrophoresis) |
(→Results of extraction : Electrophoresis) |
||
| Line 25: | Line 25: | ||
|align=center|'''Exp Size''' | |align=center|'''Exp Size''' | ||
|align=center|'''Mea Size ''' | |align=center|'''Mea Size ''' | ||
| + | |align=center|'''Mass (ng)''' | ||
|align=center|'''Gel''' | |align=center|'''Gel''' | ||
|align=center|'''Band''' | |align=center|'''Band''' | ||
| Line 36: | Line 37: | ||
! rowspan="2"| 2057 pb | ! rowspan="2"| 2057 pb | ||
! rowspan="1"| | ! rowspan="1"| | ||
| + | ! rowspan="1"| | ||
! rowspan="1"| | ! rowspan="1"| | ||
! rowspan="1"| | ! rowspan="1"| | ||
| Line 41: | Line 43: | ||
|align=center|2 | |align=center|2 | ||
! rowspan="1"| | ! rowspan="1"| | ||
| + | ! rowspan="1"| | ||
! rowspan="1"| | ! rowspan="1"| | ||
|- | |- | ||
| Line 49: | Line 52: | ||
|align=center| 2076 pb | |align=center| 2076 pb | ||
|align=center| | |align=center| | ||
| - | |align=center| 1 | + | |align=center| |
| - | |align=center| | + | ! rowspan="1"| |
| + | |align=center| | ||
|- | |- | ||
|align=center|4 | |align=center|4 | ||
| Line 57: | Line 61: | ||
! rowspan="2"| BV | ! rowspan="2"| BV | ||
! rowspan="2"| 2077 pb | ! rowspan="2"| 2077 pb | ||
| - | ! rowspan="2"| | + | ! rowspan="2"| |
| - | |align=center| | + | ! rowspan="1"| |
| - | |align=center| | + | |align=center| |
| + | |align=center| | ||
|- | |- | ||
|align=center|5 | |align=center|5 | ||
| - | |align=center| | + | |align=center| |
| - | |align=center| | + | |align=center| |
| + | ! rowspan="1"| | ||
|- | |- | ||
! rowspan="2" style="background: #ccccff;" | D101 | ! rowspan="2" style="background: #ccccff;" | D101 | ||
| Line 72: | Line 78: | ||
! rowspan="2"| BV | ! rowspan="2"| BV | ||
! rowspan="2"| 2100 pb | ! rowspan="2"| 2100 pb | ||
| - | ! rowspan="2"| | + | ! rowspan="2"| |
| - | |align=center| | + | ! rowspan="1"| |
| - | |align=center| | + | |align=center| |
| + | |align=center| | ||
|- | |- | ||
|align=center|2 | |align=center|2 | ||
|align=center| | |align=center| | ||
| - | |align=center| | + | |align=center| |
| + | ! rowspan="1"| | ||
|- | |- | ||
! rowspan="2" style="background: #ccccff;" | D102 | ! rowspan="2" style="background: #ccccff;" | D102 | ||
| Line 89: | Line 97: | ||
! rowspan="2"| | ! rowspan="2"| | ||
! rowspan="1"| | ! rowspan="1"| | ||
| + | ! rowspan="1"| | ||
! rowspan="1"| | ! rowspan="1"| | ||
|- | |- | ||
|align=center|2 | |align=center|2 | ||
! rowspan="1"| | ! rowspan="1"| | ||
| + | ! rowspan="1"| | ||
! rowspan="1"| | ! rowspan="1"| | ||
|- | |- | ||
| Line 103: | Line 113: | ||
! rowspan="2"| 2222 pb | ! rowspan="2"| 2222 pb | ||
! rowspan="2"| | ! rowspan="2"| | ||
| - | |align=center| | + | |align=center| |
| + | ! rowspan="1"| | ||
|align=center| | |align=center| | ||
|- | |- | ||
|align=center|2 | |align=center|2 | ||
|align=center| | |align=center| | ||
| - | |align=center| | + | |align=center| |
| + | ! rowspan="1"| | ||
|- | |- | ||
! rowspan="2" style="background: #ccccff;" | D104 | ! rowspan="2" style="background: #ccccff;" | D104 | ||
| Line 118: | Line 130: | ||
! rowspan="2"| 2119 pb | ! rowspan="2"| 2119 pb | ||
! rowspan="2"| | ! rowspan="2"| | ||
| - | |align=center| | + | |align=center| |
| + | ! rowspan="1"| | ||
|align=center| | |align=center| | ||
|- | |- | ||
|align=center|2 | |align=center|2 | ||
|align=center| | |align=center| | ||
| - | |align=center| | + | |align=center| |
| + | ! rowspan="1"| | ||
|- | |- | ||
! rowspan="4" style="background: #ccccff;" | D105 | ! rowspan="4" style="background: #ccccff;" | D105 | ||
| Line 132: | Line 146: | ||
! rowspan="1"| BV | ! rowspan="1"| BV | ||
! rowspan="1"| 2750 pb | ! rowspan="1"| 2750 pb | ||
| - | |align=center| | + | |align=center| |
| + | ! rowspan="1"| | ||
|align=center| | |align=center| | ||
|align=center| | |align=center| | ||
| Line 143: | Line 158: | ||
! rowspan="2"| | ! rowspan="2"| | ||
|align=center| | |align=center| | ||
| - | |align=center| | + | |align=center| |
| + | ! rowspan="1"| | ||
|- | |- | ||
|align=center|3 | |align=center|3 | ||
|align=center| | |align=center| | ||
| - | |align=center| | + | |align=center| |
| + | ! rowspan="1"| | ||
|- | |- | ||
|align=center|4 | |align=center|4 | ||
| Line 155: | Line 172: | ||
|align=center| 708 pb | |align=center| 708 pb | ||
|align=center| | |align=center| | ||
| - | |align=center| | + | |align=center| |
| + | ! rowspan="1"| | ||
|align=center| | |align=center| | ||
|- | |- | ||
| Line 167: | Line 185: | ||
|align=center| | |align=center| | ||
|align=center| | |align=center| | ||
| - | |align=center| | + | |align=center| |
| + | ! rowspan="1"| | ||
|- | |- | ||
|align=center|2 | |align=center|2 | ||
| Line 176: | Line 195: | ||
! rowspan="2"| | ! rowspan="2"| | ||
|align=center| | |align=center| | ||
| - | |align=center| | + | |align=center| |
| + | ! rowspan="1"| | ||
|- | |- | ||
|align=center|3 | |align=center|3 | ||
|align=center| | |align=center| | ||
| - | |align=center| | + | |align=center| |
| + | ! rowspan="1"| | ||
|- | |- | ||
|align=center|4 | |align=center|4 | ||
| Line 189: | Line 210: | ||
|align=center| | |align=center| | ||
|align=center| | |align=center| | ||
| + | ! rowspan="1"| | ||
|align=center| | |align=center| | ||
|- | |- | ||
| Line 199: | Line 221: | ||
|align=center| 779 pb | |align=center| 779 pb | ||
|align=center| | |align=center| | ||
| - | |align=center| | + | |align=center| |
| + | ! rowspan="1"| | ||
|align=center| | |align=center| | ||
|- | |- | ||
| Line 209: | Line 232: | ||
|align=center| | |align=center| | ||
|align=center| | |align=center| | ||
| - | |align=center| | + | |align=center| |
| + | ! rowspan="1"| | ||
|- | |- | ||
! rowspan="2" style="background: #ccccff;"|D108 | ! rowspan="2" style="background: #ccccff;"|D108 | ||
| Line 220: | Line 244: | ||
|align=center| | |align=center| | ||
|align=center| | |align=center| | ||
| - | |align=center| | + | |align=center| |
| + | ! rowspan="1"| | ||
|- | |- | ||
|align=center| 2 | |align=center| 2 | ||
| Line 228: | Line 253: | ||
|align=center| 745 pb | |align=center| 745 pb | ||
|align=center| | |align=center| | ||
| - | |align=center| | + | |align=center| |
| + | ! rowspan="1"| | ||
|align=center| | |align=center| | ||
|- | |- | ||
| Line 240: | Line 266: | ||
|align=center| | |align=center| | ||
|align=center| | |align=center| | ||
| - | |align=center| | + | |align=center| |
| + | ! rowspan="1"| | ||
|- | |- | ||
|align=center| 2 | |align=center| 2 | ||
| Line 248: | Line 275: | ||
|align=center| 745 pb | |align=center| 745 pb | ||
|align=center| | |align=center| | ||
| - | |align=center| | + | |align=center| |
| + | ! rowspan="1"| | ||
|align=center| | |align=center| | ||
|- | |- | ||
| Line 259: | Line 287: | ||
|align=center| 743 pb | |align=center| 743 pb | ||
|align=center| | |align=center| | ||
| - | |align=center| | + | |align=center| |
| + | ! rowspan="1"| | ||
|align=center| | |align=center| | ||
|- | |- | ||
| Line 268: | Line 297: | ||
|align=center| 742 pb | |align=center| 742 pb | ||
|align=center| | |align=center| | ||
| - | |align=center| | + | |align=center| |
| + | ! rowspan="1"| | ||
|align=center| | |align=center| | ||
|- | |- | ||
| Line 280: | Line 310: | ||
|align=center| | |align=center| | ||
|align=center| | |align=center| | ||
| - | |align=center| | + | |align=center| |
| + | ! rowspan="1"| | ||
|- | |- | ||
|align=center| 2 | |align=center| 2 | ||
| Line 289: | Line 320: | ||
|align=center| | |align=center| | ||
|align=center| | |align=center| | ||
| - | |align=center| | + | |align=center| |
| + | ! rowspan="1"| | ||
|- | |- | ||
! rowspan="2" style="background: #ccccff;"|D116 | ! rowspan="2" style="background: #ccccff;"|D116 | ||
| Line 299: | Line 331: | ||
! rowspan="2"| 2100 pb | ! rowspan="2"| 2100 pb | ||
! rowspan="2"| | ! rowspan="2"| | ||
| + | ! rowspan="1"| | ||
! rowspan="1"| | ! rowspan="1"| | ||
! rowspan="1"| | ! rowspan="1"| | ||
| Line 304: | Line 337: | ||
|align=center|2 | |align=center|2 | ||
! rowspan="1"| | ! rowspan="1"| | ||
| + | ! rowspan="1"| | ||
! rowspan="1"| | ! rowspan="1"| | ||
|- | |- | ||
| Line 314: | Line 348: | ||
! rowspan="2"| 2100 pb | ! rowspan="2"| 2100 pb | ||
! rowspan="2"| | ! rowspan="2"| | ||
| + | ! rowspan="1"| | ||
! rowspan="1"| | ! rowspan="1"| | ||
! rowspan="1"| | ! rowspan="1"| | ||
| Line 319: | Line 354: | ||
|align=center|2 | |align=center|2 | ||
! rowspan="1"| | ! rowspan="1"| | ||
| + | ! rowspan="1"| | ||
! rowspan="1"| | ! rowspan="1"| | ||
|- | |- | ||
| Line 330: | Line 366: | ||
! rowspan="2"| | ! rowspan="2"| | ||
! rowspan="1"| | ! rowspan="1"| | ||
| + | ! rowspan="1"| | ||
! rowspan="1"| | ! rowspan="1"| | ||
|- | |- | ||
|align=center|2 | |align=center|2 | ||
! rowspan="1"| | ! rowspan="1"| | ||
| + | ! rowspan="1"| | ||
! rowspan="1"| | ! rowspan="1"| | ||
|- | |- | ||
| Line 344: | Line 382: | ||
! rowspan="2"| 5621 pb | ! rowspan="2"| 5621 pb | ||
! rowspan="2"| | ! rowspan="2"| | ||
| + | ! rowspan="1"| | ||
! rowspan="1"| | ! rowspan="1"| | ||
! rowspan="1"| | ! rowspan="1"| | ||
| Line 349: | Line 388: | ||
|align=center|2 | |align=center|2 | ||
! rowspan="1"| | ! rowspan="1"| | ||
| + | ! rowspan="1"| | ||
! rowspan="1"| | ! rowspan="1"| | ||
|- | |- | ||
| Line 359: | Line 399: | ||
! rowspan="2"| 37 pb | ! rowspan="2"| 37 pb | ||
! rowspan="2"| | ! rowspan="2"| | ||
| + | ! rowspan="1"| | ||
! rowspan="1"| | ! rowspan="1"| | ||
! rowspan="1"| | ! rowspan="1"| | ||
| Line 364: | Line 405: | ||
|align=center|2 | |align=center|2 | ||
! rowspan="1"| | ! rowspan="1"| | ||
| + | ! rowspan="1"| | ||
! rowspan="1"| | ! rowspan="1"| | ||
|- | |- | ||
| Line 372: | Line 414: | ||
! rowspan="1"| 2079 pb | ! rowspan="1"| 2079 pb | ||
! rowspan="1"| | ! rowspan="1"| | ||
| + | ! rowspan="1"| | ||
! rowspan="1"| | ! rowspan="1"| | ||
! rowspan="1"| | ! rowspan="1"| | ||
| Line 381: | Line 424: | ||
! rowspan="2"| 2080 pb | ! rowspan="2"| 2080 pb | ||
! rowspan="2"| | ! rowspan="2"| | ||
| + | ! rowspan="1"| | ||
! rowspan="1"| | ! rowspan="1"| | ||
! rowspan="1"| | ! rowspan="1"| | ||
| Line 386: | Line 430: | ||
|align=center|5 | |align=center|5 | ||
! rowspan="1"| | ! rowspan="1"| | ||
| + | ! rowspan="1"| | ||
! rowspan="1"| | ! rowspan="1"| | ||
|- | |- | ||
| Line 396: | Line 441: | ||
! rowspan="3"| 939 pb | ! rowspan="3"| 939 pb | ||
! rowspan="3"| | ! rowspan="3"| | ||
| + | ! rowspan="1"| | ||
! rowspan="1"| | ! rowspan="1"| | ||
! rowspan="1"| | ! rowspan="1"| | ||
| Line 401: | Line 447: | ||
|align=center|2 | |align=center|2 | ||
! rowspan="1"| | ! rowspan="1"| | ||
| + | ! rowspan="1"| | ||
! rowspan="1"| | ! rowspan="1"| | ||
|- | |- | ||
|align=center|3 | |align=center|3 | ||
! rowspan="1"| | ! rowspan="1"| | ||
| + | ! rowspan="1"| | ||
! rowspan="1"| | ! rowspan="1"| | ||
|- | |- | ||
| Line 415: | Line 463: | ||
! rowspan="3"| 900 pb | ! rowspan="3"| 900 pb | ||
! rowspan="1"| | ! rowspan="1"| | ||
| + | ! rowspan="1"| | ||
! rowspan="1"| | ! rowspan="1"| | ||
! rowspan="1"| | ! rowspan="1"| | ||
| Line 420: | Line 469: | ||
|align=center|2 | |align=center|2 | ||
! rowspan="1"| | ! rowspan="1"| | ||
| + | ! rowspan="1"| | ||
! rowspan="1"| | ! rowspan="1"| | ||
|- | |- | ||
|align=center|3 | |align=center|3 | ||
! rowspan="1"| | ! rowspan="1"| | ||
| + | ! rowspan="1"| | ||
! rowspan="1"| | ! rowspan="1"| | ||
|} | |} | ||
Revision as of 14:52, 28 July 2008
Results of extraction : Electrophoresis
conditions :
- 10µl of ladder 1 kb
- 2µl of extraction added with 2µl of loading Dye 6x
- migration ~30min at 100W
- Gel 1, 2, 3, 4, 5 = 0.8%
- Gel 6 = 1,5%
gel1 
gel2 
gel3 
| Name | BioBrick | Tube N° | Enz 1 | Enz 2 | Obs | Exp Size | Mea Size | Mass (ng) | Gel | Band |
| D100 | B0034 | 1 | XbaI | PstI | BI | 2057 pb | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| 2 | ||||||||||
| 3 | EcoRI | XbaI | FV | 2076 pb | ||||||
| 4 | SpeI | PstI | BV | 2077 pb | ||||||
| 5 | ||||||||||
| D101 | J23101 | 1 | SpeI | PstI | BV | 2100 pb | ||||
| 2 | ||||||||||
| D102 | J23109 | 1 | SpeI | PstI | BV | 2100 pb | ||||
| 2 | ||||||||||
| D103 | R0079 | 1 | SpeI | PstI | BV | 2222 pb | ||||
| 2 | ||||||||||
| D104 | R0040 | 1 | SpeI | PstI | BV | 2119 pb | ||||
| 2 | ||||||||||
| D105 | S03154 | 1 | SpeI | PstI | BV | 2750 pb | ||||
| 2 | XbaI | PstI | BI | 707 pb | ||||||
| 3 | ||||||||||
| 4 | EcoRI | SpeI | FI | 708 pb | ||||||
| D106 | S03879 | 1 | SpeI | PstI | BV | 2768 pb | ||||
| 2 | XbaI | PstI | BI | 725 pb | ||||||
| 3 | ||||||||||
| 4 | EcoRI | SpeI | FI | 726 pb | ||||||
| D107 | C0079 | 1 | EcoRI | SpeI | FI | 779 pb | ||||
| 2 | XbaI | PstI | BI | 778 pb | ||||||
| D108 | C0179 | 1 | EcoRI | SpeI | FI | 746 pb | ||||
| 2 | XbaI | PstI | BI | 745 pb | ||||||
| D109 | E0030 | 1 | EcoRI | SpeI | FI | 746 pb | ||||
| 2 | XbaI | PstI | BI | 745 pb | ||||||
| D110 | E0040 | 1 | EcoRI | SpeI | FI | 743 pb | ||||
| 2 | XbaI | PstI | BI | 742 pb | ||||||
| D111 | E1010 | 1 | EcoRI | SpeI | FI | 704 pb | ||||
| 2 | XbaI | PstI | BI | 703 pb | ||||||
| D116 | J23100 | 1 | SpeI | PstI | BV | 2100 pb | ||||
| 2 | ||||||||||
| D117 | J23107 | 1 | SpeI | PstI | BV | 2100 pb | ||||
| 2 | ||||||||||
| D118 | B0015 | 1 | EcoRI | XbaI | FV | 3303 pb | ||||
| 2 | ||||||||||
| D119 | I0500 | 1 | SpeI | PstI | FV | 5621 pb | ||||
| 2 | ||||||||||
| D120 | B0030 | 1 | XbaI | PstI | BI | 37 pb | ||||
| 2 | ||||||||||
| 3 | EcoRI | XbaI | FV | 2079 pb | ||||||
| 4 | SpeI | PstI | BV | 2080 pb | ||||||
| 5 | ||||||||||
| D121 | E0422 | 1 | XbaI | PstI | BI | 939 pb | ||||
| 2 | ||||||||||
| 3 | ||||||||||
| D122 | E0840 | 1 | XbaI | PstI | BI | 900 pb | ||||
| 2 | ||||||||||
| 3 |
==> conclusion :
