Team:Paris/Modeling/More f3 Table

From 2008.igem.org

(Difference between revisions)
Line 48: Line 48:
|-
|-
|β<sub>22</sub>
|β<sub>22</sub>
-
|total transcription rate of ''FliA''><''pFlhDC'' '''with RBS B0034''' <br> β<sub>22</sub>
+
|total transcription rate of <br> ''FliA''><''pFlhDC'' '''with RBS B0034''' <br> β<sub>22</sub>
|nM.min<sup>-1</sup>
|nM.min<sup>-1</sup>
|
|
|
|
|-
|-
-
|(K<sub>6</sub>/{coef<sub>fliA</sub>})
+
|(K<sub>6</sub>/{coef<sub>''fliA''</sub>})
|activation constant of ''FliA''><''pFlhDC'' <br> K<sub>6</sub>
|activation constant of ''FliA''><''pFlhDC'' <br> K<sub>6</sub>
|nM
|nM
Line 66: Line 66:
|-
|-
|β<sub>17</sub>
|β<sub>17</sub>
-
|basal activity of ''pFlhDC'' '''with RBS B0034''' <br> β<sub>17</sub>
+
|basal activity of <br> ''pFlhDC'' '''with RBS B0034''' <br> β<sub>17</sub>
|nM.min<sup>-1</sup>
|nM.min<sup>-1</sup>
|
|
|
|
|-
|-
-
|(K<sub>15</sub>/{coef<sub>omp</sub>})
+
|(K<sub>15</sub>/{coef<sub>''ompR''</sub>})
|activation constant of ''OmpR''><''pFlhDC'' <br> K<sub>15</sub>
|activation constant of ''OmpR''><''pFlhDC'' <br> K<sub>15</sub>
|nM
|nM

Revision as of 13:00, 29 October 2008

param signification unit value comments
(fluorescence) value of the observed fluorescence au need for 20 mesures with well choosen values of [aTc]i
and for 20 mesures with well choosen values of [arab]i
and 5x5 measures for the relation below?
conversion conversion ratio between
fluorescence and concentration
↓ gives ↓
nM.au-1 (1/79.429)
[GFP] GFP concentration at steady-state nM
γGFP dilution-degradation rate
of GFP(mut3b)
↓ gives ↓
min-1 0.0198 Time Cell Division : 35 min.
ƒ3 activity of
pFlhDC with RBS E0032
nM.min-1



param signification
corresponding parameters in the equations
unit value comments
β22 total transcription rate of
FliA><pFlhDC with RBS B0034
β22
nM.min-1
(K6/{coeffliA}) activation constant of FliA><pFlhDC
K6
nM
n6 complexation order of FliA><pFlhDC
n6
no dimension
β17 basal activity of
pFlhDC with RBS B0034
β17
nM.min-1
(K15/{coefompR}) activation constant of OmpR><pFlhDC
K15
nM
n15 complexation order of OmpR><pFlhDC
n15
no dimension