Team:Paris/Modeling/Workflow Example

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Generic Workflow on an Example

Here, we will explain the generic protocole that leads to the caracterisation of one of the steps of the system. The choosen example is the EXPRESSION of the PROMOTER pFliL in function of the TRANSCRIPTION FACTORS FlhDC and FliA. We know that the hexamere FlhD4C2 and the protein FliA act both on the promoter pFliL as inducers... how do we evaluate this action ?

Steps
Corresponding Hypothesis and Methods
First, we consider the complexations FlhDC><pFliL and FlhDC><pFliL : FlhD4C2 → FlhDC

Quasi Steady-State
"Hill Type" complexation

File:Complexation.jpg
Then, we consider the resulting activation of pFliL : Transduction Rate β

"Sum Logical Gate"

File:Activation
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