# Team:Paris/Modeling/f0

(Difference between revisions)
 Revision as of 08:39, 29 October 2008 (view source)Hugo (Talk | contribs)← Older edit Latest revision as of 02:04, 30 October 2008 (view source)Hugo (Talk | contribs) (15 intermediate revisions not shown) Line 1: Line 1: {{Paris/Menu}} {{Paris/Menu}} - {{Paris/Header|Method & Algorithm : ƒ0}} + {{Paris/Header|Method & Algorithm : ƒ0 }} +
= act_J23101
+
- [[Image:F0.jpg|thumb|Specific Plasmid Characterisation for ƒ0]] In the '''exponential phase of growth''', at the steady state, we have + [[Image:F0.jpg|thumb|Specific Plasmid Characterisation for ƒ0]] + + According to the characterization plasmid (see right) and to our modeling, in the '''exponential phase of growth''', at the steady state, the experiment would give us [[Image:eqF0.jpg]] [[Image:eqF0.jpg]] + +
+ {{Paris/Toggle|Table of Values|Team:Paris/Modeling/More_f0}} +
+ + +
That will directly give us ƒ0
+ +
+
- {|border="1" style="text-align: center" + [[Team:Paris/Modeling/Characterization_Approach| - |param + [[Team:Paris/Modeling/Characterization_Approach| J23101 '''with RBS E0032''' + - |nM.min-1 + - | + - | + - |- + - |γGFP + - |dilution-degradation rate
of GFP(mut3b) + - |min-1 + - | + - |- + - |[GFP] + - |GFP concentration at steady-state + - |nM + - | + - | + - |- + - |(''fluorescence'') + - |value of the observed fluorescence + - |au + - | + - |mean value of ~20 measures + - |- + - |''conversion'' + - |conversion ratio between
fluorescence and concentration + - |nM.au-1 + - |(1/79.429) + - | + - |} +
- - That will directly give us ƒ0, which corresponds to β72 in the [[Team:Paris/Modeling/Synchronisation|equations]], if the ''pConstitutive'' is J23101.

## Latest revision as of 02:04, 30 October 2008

Method & Algorithm : ƒ0

= act_J23101

Specific Plasmid Characterisation for ƒ0

According to the characterization plasmid (see right) and to our modeling, in the exponential phase of growth, at the steady state, the experiment would give us

↓ Table of Values ↑

 param signification unit value comments (fluorescence) value of the observed fluorescence au mean value of ~20 measures conversion conversion ratio between fluorescence and concentration ↓ gives ↓ nM.au-1 (1/79.429) [GFP] GFP concentration at steady-state nM γGFP dilution-degradation rate of GFP(mut3b) ↓ gives ↓ min-1 0.0198 Only dilution :Time Cell Division : 35 min. ƒ0 activity of J23101 with RBS E0032 nM.min-1

That will directly give us ƒ0