# Team:Paris/Modeling/f0

(Difference between revisions)
 Revision as of 11:16, 19 October 2008 (view source)Hugo (Talk | contribs)← Older edit Revision as of 08:38, 29 October 2008 (view source)Hugo (Talk | contribs) Newer edit → Line 1: Line 1: - [[Image:F0.jpg|thumb]] In the '''exponential phase of growth''', at the steady state, we have + {{Paris/Menu}} - [[Image:eqF0.jpg]] + {{Paris/Header|Method & Algorithm : ƒ0}} + [[Image:F0.jpg|thumb|Specific Plasmid Characterisation for ƒ0]] In the '''exponential phase of growth''', at the steady state, we have + [[Image:eqF0.jpg]] + +
{|border="1" style="text-align: center" {|border="1" style="text-align: center" |param |param Line 9: Line 13: |unit |unit |value |value + |comments |- |- |[exprJ23101] |[exprJ23101] |expression rate of
J23101 '''with RBS E0032''' |expression rate of
J23101 '''with RBS E0032''' |nM.min-1 |nM.min-1 + | | | |- |- Line 18: Line 24: |dilution-degradation rate
of GFP(mut3b) |min-1 |min-1 - |0.0198 + | |- |- |[GFP] |[GFP] Line 35: Line 41: |(1/79.429) |(1/79.429) |} |} +
That will directly give us ƒ0, which corresponds to β72 in the [[Team:Paris/Modeling/Synchronisation|equations]], if the ''pConstitutive'' is J23101. That will directly give us ƒ0, which corresponds to β72 in the [[Team:Paris/Modeling/Synchronisation|equations]], if the ''pConstitutive'' is J23101.

## Revision as of 08:38, 29 October 2008

Method & Algorithm : ƒ0

Specific Plasmid Characterisation for ƒ0
In the exponential phase of growth, at the steady state, we have

 param signification unit value comments [exprJ23101] expression rate of J23101 with RBS E0032 nM.min-1 γGFP dilution-degradation rate of GFP(mut3b) min-1 [GFP] GFP concentration at steady-state nM (fluorescence) value of the observed fluorescence au mean value of ~20 measures conversion conversion ratio between fluorescence and concentration nM.au-1 (1/79.429)

That will directly give us ƒ0, which corresponds to β72 in the equations, if the pConstitutive is J23101.