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Method & Algorithm : ƒ0

Specific Plasmid Characterisation for ƒ0
In the exponential phase of growth, at the steady state, we have


param signification unit value comments
[exprJ23101] expression rate of
J23101 with RBS E0032
γGFP dilution-degradation rate
of GFP(mut3b)
min-1 0.0198 Only dilution :
Time Cell Division : 35 min.
[GFP] GFP concentration at steady-state nM
(fluorescence) value of the observed fluorescence au mean value of ~20 measures
conversion conversion ratio between
fluorescence and concentration (1/79.429)

That will directly give us ƒ0