Team:Paris/Modeling/f1

From 2008.igem.org

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No particular strain.
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[[Image:f2.jpg]]
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[[Image:f2alt.png]]
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To deduce from our measurements the correct hill function, see the [[Team:Paris/Modeling/estimation#how_to_control_the_concentration_of_the_transcription_factor_.3F|different methods]].
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The best one consist in finding in the literature the dissociation constant of the reaction aTc + TetR&#8644; aTc_TetR, in order to determine by calculus the amount of free TetR, function of the expression of a known constitutive promoter before ''tetR'' and of the added aTc. In this way, we just have to study [expr.''Ptet''] = &#131;1bis(TetR<sub>free</sub>).
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Revision as of 15:47, 18 August 2008

F2.jpg