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Team:Paris/Modeling/f1
From 2008.igem.org
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| - | [[Image:f1a.jpg|thumb]] | + | [[Image:f1a.jpg|thumb]] In the '''exponential phase of growth''', at the steady state, we have |
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| + | <center>[[Image:tetReqtot.jpg]]</center> | ||
| + | |||
| + | so, with | ||
| + | |||
| + | <center>[[Image:f1expr.jpg]]</center> | ||
| + | |||
| + | that gives, after reducing the expression | ||
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| + | <center>[[Image:f1exprredu.jpg]]</center> | ||
| + | |||
| + | with the previous expression of [TetR]<sub>eq</sub> | ||
| + | |||
| + | <center>[[Image:f1exprreduf0.jpg]]</center> | ||
| + | |||
| + | so, finally, if we use a ''calculated'' value of [TetR] (by using the expression of ƒ0 for instance, or by introducing a value given by ''tetR'' after an other "caracterised promoter") | ||
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| + | <center>[[Image:f1exprCalc.jpg]]</center> | ||
| + | |||
| + | |||
| + | {|border="1" style="text-align: center" | ||
| + | |param | ||
| + | |signification | ||
| + | |unit | ||
| + | |value | ||
| + | |- | ||
| + | |[exprJ23101] | ||
| + | |expression rate of <br> J23101 '''with RBS E0032''' | ||
| + | |nM.s<sup>-1</sup> | ||
| + | | | ||
| + | |- | ||
| + | |γ<sub>GFP</sub> | ||
| + | |dilution-degradation rate <br> of GFP(mut3b) | ||
| + | |s<sup>-1</sup> | ||
| + | |ln(2)/3600 | ||
| + | |- | ||
| + | |[GFP] | ||
| + | |GFP concentration at steady-state | ||
| + | |nM | ||
| + | | | ||
| + | |- | ||
| + | |(''fluorescence'') | ||
| + | |value of the observed fluorescence | ||
| + | |au | ||
| + | | | ||
| + | |- | ||
| + | |''conversion'' | ||
| + | |conversion ration between <br> fluorescence and concentration | ||
| + | |nM.au<sup>-1</sup> | ||
| + | |(1/79.429) | ||
| + | |} | ||
Revision as of 23:53, 20 August 2008
so, with
that gives, after reducing the expression
with the previous expression of [TetR]eq
so, finally, if we use a calculated value of [TetR] (by using the expression of ƒ0 for instance, or by introducing a value given by tetR after an other "caracterised promoter")
| param | signification | unit | value |
| [exprJ23101] | expression rate of J23101 with RBS E0032 | nM.s-1 | |
| γGFP | dilution-degradation rate of GFP(mut3b) | s-1 | ln(2)/3600 |
| [GFP] | GFP concentration at steady-state | nM | |
| (fluorescence) | value of the observed fluorescence | au | |
| conversion | conversion ration between fluorescence and concentration | nM.au-1 | (1/79.429) |
