Team:Paris/Modeling/f1

From 2008.igem.org

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|(&gamma; K<sub>tet</sub>/{coef<sub>tetR</sub>} &131;)
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|(&gamma; K<sub>tet</sub> / {coef<sub>tetR</sub>} &#131;0)
|activation constant of pTet <br> K<sub>18</sub>
|activation constant of pTet <br> K<sub>18</sub>
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Revision as of 09:59, 8 October 2008

F1a.jpg

The experience would give us

F1expr.jpg

Thus, at steady-state and in the exponential phase of growth :

ExprptetF0.jpg


param signification unit value
[expr(pTet)] expression rate of
pTet with RBS E0032
nM.min-1 see "findparam"
need for 20 measures
γGFP dilution-degradation rate
of GFP(mut3b)
min-1 0.0198
[GFP] GFP concentration at steady-state nM need for 20 measures
(fluorescence) value of the observed fluorescence au need for 20 measures
conversion conversion ration between
fluorescence and concentration
nM.au-1 (1/79.429)



param signification
corresponding parameters in the equations
unit value
βtet production rate of pTet with RBS E0032
β1
nM.min-1
(γ Ktet / {coeftetR} ƒ0) activation constant of pTet
K18
nM
ntet complexation order of pTet
n18
no dimension
KaTc complexation constant aTc-TetR
K17
nM
naTc complexation order aTc-TetR
n17
no dimension

Also, this experiment will allow us to know the expression of ƒ1 :

ExprF1.jpg