Team:Paris/Modeling/f2

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F2.jpg

The experience would give us

F2expr.jpg

Thus, at steady-state and in the exponential phase of growth :

Exprpbad.jpg
param signification unit value
[expr(pBad)] expression rate of
pBad with RBS E0032
nM.min-1 see "findparam"
need for 20 measures
γGFP dilution-degradation rate
of GFP(mut3b)
min-1 0.0198
[GFP] GFP concentration at steady-state nM need for 20 measures
(fluorescence) value of the observed fluorescence au need for 20 measures
conversion conversion ratio between
fluorescence and concentration
nM.au-1 (1/79.429)



param signification
corresponding parameters in the equations
unit value
βbad production rate of pBad with RBS E0032
not in the system
nM.min-1
(Kbad/[AraCtot]) activation constant of pBad
not in the system
nM
nbad complexation order of pBad
not in the system
no dimension
Kara complexation constant Arabinose-AraC
not in the system
nM
nara complexation order Arabinose-AraC
not in the system
no dimension

That will give us directly ƒ2([arab])