"
Team:Paris/Modeling/f3
From 2008.igem.org
(Difference between revisions)
| Line 1: | Line 1: | ||
[[Image:f3omp.jpg|thumb]] | [[Image:f3omp.jpg|thumb]] | ||
| - | We have [OmpR<sup>*</sup>] = {coef}<sub>OmpR</sub>''expr(pTet)'' = {coef}<sub>OmpR</sub> #131;1([aTc]<sub>i</sub>) | + | We have [OmpR<sup>*</sup>] = {coef}<sub>OmpR</sub>''expr(pTet)'' = {coef}<sub>OmpR</sub> ƒ1([aTc]<sub>i</sub>) |
| - | and [FliA] = {coef}<sub>FliA</sub>''expr(pBad)'' = {coef}<sub>FliA</sub> #131;2([arab]<sub>i</sub>) | + | and [FliA] = {coef}<sub>FliA</sub>''expr(pBad)'' = {coef}<sub>FliA</sub> ƒ2([arab]<sub>i</sub>) |
So, at steady-states, | So, at steady-states, | ||
| - | [[Image:F3ompfinal.jpg | + | [[Image:F3ompfinal.jpg|center]] |
<br><br> | <br><br> | ||
Revision as of 12:00, 12 October 2008
We have [OmpR*] = {coef}OmpRexpr(pTet) = {coef}OmpR ƒ1([aTc]i)
and [FliA] = {coef}FliAexpr(pBad) = {coef}FliA ƒ2([arab]i)
So, at steady-states,
| param | signification | unit | value | comments |
| [expr(pFlhDC)] | expression rate of pFlhDC with RBS E0032 | nM.min-1 | need for 20 mesures with well choosen values of [aTc]i and for 20 mesures with well choosen values of [arab]i and 5x5 measures for the SUM? | |
| γGFP | dilution-degradation rate of GFP(mut3b) | min-1 | 0.0198 | |
| [GFP] | GFP concentration at steady-state | nM | need for 20 + 20 measures and 5x5 measures for the SUM? | |
| (fluorescence) | value of the observed fluorescence | au | need for 20 + 20 measures and 5x5 measures for the SUM? | |
| conversion | conversion ratio between fluorescence and concentration | nM.au-1 | (1/79.429) |
| param | signification corresponding parameters in the equations | unit | value | comments |
| β12 | production rate of FliA-pFlhDC with RBS E0032 β12 | nM.min-1 | ||
| (K11/{coeffliA}) | activation constant of FliA-pFlhDC K11 | nM | ||
| n11 | complexation order of FliA-pFlhDC n11 | no dimension | ||
| β2 | production rate of OmpR-pFlhDC with RBS E0032 β2 | nM.min-1 | ||
| (K19/{coefompR}) | activation constant of OmpR-pFlhDC K19 | nM | ||
| n19 | complexation order of OmpR-pFlhDC n19 | no dimension |
Then, if we have time, we want to verify the expected relation
