Team:Paris/Modeling/f3

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[[Image:f3omp.jpg|thumb]]
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[[Image:f3omp.jpg|thumb]] At the steady-state, we have
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<center>[[Image:FliAeq.jpg]]</center>
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and
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<center>[[Image:OmpReq.jpg]]</center>
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so the expression
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<center>[[Image:f3expr.jpg]]</center>
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gives
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<center>[[Image:f3FliA.jpg]]</center>
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and
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<center>[[Image:f3OmpR.jpg]]</center>
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and for ''calculated values of the TF'',
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<center>[[Image:f3FliACalc.jpg]]</center>
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and
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<center>[[Image:f3OmpRCalc.jpg]]</center>
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{|border="1" style="text-align: center"
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|param
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|signification
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|unit
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|value
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|-
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|[expr(pFlhDC)]
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|expression rate of <br> pFlhDC '''with RBS E0032'''
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|nM.s<sup>-1</sup>
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|see [[Team:Paris/Modeling/Programs|"findparam"]] <br> need for 20 + 20 measures <br> and 5x5 measures for the ''SUM''?
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|-
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|γ<sub>GFP</sub>
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|dilution-degradation rate <br> of GFP(mut3b)
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|s<sup>-1</sup>
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|ln(2)/3600
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|-
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|[GFP]
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|GFP concentration at steady-state
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|nM
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|need for 20 measures
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|-
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|(''fluorescence'')
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|value of the observed fluorescence
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|au
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|need for 20 + 20 measures <br> and 5x5 measures for the ''SUM''?
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|-
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|''conversion''
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|conversion ration between <br> fluorescence and concentration
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|nM.au<sup>-1</sup>
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|(1/79.429)
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|}
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<br><br>
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{|border="1" style="text-align: center"
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|param
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|signification <br> corresponding parameters in the [[Team:Paris/Modeling/Oscillations#Resulting_Equations|equations]]
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|unit
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|value
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|-
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|β<sub>bad</sub>
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|production rate of pFlhDC '''with RBS E0032''' <br> not in the system
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|nM.s<sup>-1</sup>
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|
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|-
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|(K<sub>bad</sub>/[AraC<sub>tot</sub>]<sup>n<sub>bad</sub></sup>)
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|activation constant of pBad <br> not in the system
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|nM<sup>n<sub>bad</sub></sup>
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|
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|-
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|n<sub>bad</sub>
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|complexation order of pBad<br> not in the system
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|no dimension
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|
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|-
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|K<sub>ara</sub>
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|complexation constant Arabinose-AraC <br> not in the system
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|nM<sup>n<sub>ara</sub></sup>
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|
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|-
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|n<sub>ara</sub>
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|complexation order Arabinose-AraC <br> n<sub>17</sub>
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|no dimension
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|
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|}

Revision as of 14:26, 21 August 2008

F3omp.jpg
At the steady-state, we have
FliAeq.jpg

and

OmpReq.jpg

so the expression

F3expr.jpg

gives

F3FliA.jpg

and

F3OmpR.jpg

and for calculated values of the TF,

F3FliACalc.jpg

and

F3OmpRCalc.jpg
param signification unit value
[expr(pFlhDC)] expression rate of
pFlhDC with RBS E0032 		nM.s-1 see "findparam"
need for 20 + 20 measures
and 5x5 measures for the SUM?
γGFP dilution-degradation rate
of GFP(mut3b) 		s-1 ln(2)/3600
[GFP] GFP concentration at steady-state nM need for 20 measures
(fluorescence) value of the observed fluorescence au need for 20 + 20 measures
and 5x5 measures for the SUM?
conversion conversion ration between
fluorescence and concentration 		nM.au-1 (1/79.429)



param signification
corresponding parameters in the equations 		unit value
βbad production rate of pFlhDC with RBS E0032
not in the system 		nM.s-1
(Kbad/[AraCtot]nbad) activation constant of pBad
not in the system 		nMnbad
nbad complexation order of pBad
not in the system 		no dimension
Kara complexation constant Arabinose-AraC
not in the system 		nMnara
nara complexation order Arabinose-AraC
n17 		no dimension
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