"

 

From 2008.igem.org

At the steady-state, we have

and

so the expression

gives

and

and for calculated values of the TF,

and

param	signification	unit	value
[expr(pFlhDC)]	expression rate of pFlhDC with RBS E0032	nM.s-1	see "findparam" need for 20 + 20 measures and 5x5 measures for the SUM?
γGFP	dilution-degradation rate of GFP(mut3b)	s-1	ln(2)/3600
[GFP]	GFP concentration at steady-state	nM	need for 20 measures
(fluorescence)	value of the observed fluorescence	au	need for 20 + 20 measures and 5x5 measures for the SUM?
conversion	conversion ration between fluorescence and concentration	nM.au-1	(1/79.429)

param	signification corresponding parameters in the equations	unit	value
βbad	production rate of pFlhDC with RBS E0032 not in the system	nM.s-1
(Kbad/[AraCtot]nbad)	activation constant of pBad not in the system	nMnbad
nbad	complexation order of pBad not in the system	no dimension
Kara	complexation constant Arabinose-AraC not in the system	nMnara
nara	complexation order Arabinose-AraC n17	no dimension

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